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Metallic Nanofilm Enhanced Fluorescence Cell Imaging: A Study of Distance-Dependent Intensity and Lifetime by Optical Sectioning Microscopy
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2020-03-25 , DOI: 10.1021/acs.jpcb.9b11390 Yan-Yun Zhai 1 , Qian Liu 1 , Wei-Peng Cai 1 , Shuo-Hui Cao 1 , Li-Xiang Zhang 1 , Yao-Qun Li 1
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2020-03-25 , DOI: 10.1021/acs.jpcb.9b11390 Yan-Yun Zhai 1 , Qian Liu 1 , Wei-Peng Cai 1 , Shuo-Hui Cao 1 , Li-Xiang Zhang 1 , Yao-Qun Li 1
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Simple, stable, easily-fabricated smooth metallic nanofilm can improve the imaging intensity and imaging contrast. However, its application in micrometer-scale cells has not been popularized due to the lack of full understanding of their related fluorescence properties. In this study, fluorescence enhancement of cell imaging on smooth Au nanofilm was investigated over a micrometer-scale range via employment of the optical sectioning method available with a laser scanning confocal fluorescence microscope. The fluorescence enhancement reduced with the distance away from the surface of metallic nanofilm, and this distance dependence was determined by the factors of numerical aperture, dye–substrate distance, and emission wavelength. In addition, distance-dependent fluorescence lifetime images of cells were also measured to study the interaction between fluorophores and metallic film. The enhancement effect of Au nanofilm on fluorescence cell imaging can be induced not only by the standing wave formed by the reflected light and exciting light but also by the interaction between fluorophore and surface plasmons on the metallic nanofilm. Our study on smooth metallic nanofilm should pave the way for utilizing its uniform fluorescence enhancement characteristic for biological imaging.
中文翻译：
金属纳米膜增强的荧光细胞成像：光学切片显微镜对距离依赖的强度和寿命的研究。
简单，稳定，易于制造的光滑金属纳米膜可以提高成像强度和成像对比度。然而，由于对它们相关的荧光特性缺乏全面的了解，其在微米级细胞中的应用尚未普及。在这项研究中，通过使用激光扫描共聚焦荧光显微镜可用的光学切片方法，在微米级范围内研究了光滑Au纳米膜上细胞成像的荧光增强作用。荧光增强作用随着与金属纳米膜表面距离的增加而减小，并且这种距离依赖性取决于数值孔径，染料-基质距离和发射波长的因素。此外，还测量了细胞的距离依赖性荧光寿命图像，以研究荧光团与金属膜之间的相互作用。金纳米膜对荧光细胞成像的增强作用不仅可以由反射光和激发光形成的驻波来诱导，而且还可以通过荧光团与金属纳米膜上的表面等离子体激元之间的相互作用来诱导。我们对光滑的金属纳米膜的研究应为利用其均匀的荧光增强特性进行生物成像铺平道路。
更新日期：2020-03-26
中文翻译：
金属纳米膜增强的荧光细胞成像：光学切片显微镜对距离依赖的强度和寿命的研究。
简单，稳定，易于制造的光滑金属纳米膜可以提高成像强度和成像对比度。然而，由于对它们相关的荧光特性缺乏全面的了解，其在微米级细胞中的应用尚未普及。在这项研究中，通过使用激光扫描共聚焦荧光显微镜可用的光学切片方法，在微米级范围内研究了光滑Au纳米膜上细胞成像的荧光增强作用。荧光增强作用随着与金属纳米膜表面距离的增加而减小，并且这种距离依赖性取决于数值孔径，染料-基质距离和发射波长的因素。此外，还测量了细胞的距离依赖性荧光寿命图像，以研究荧光团与金属膜之间的相互作用。金纳米膜对荧光细胞成像的增强作用不仅可以由反射光和激发光形成的驻波来诱导，而且还可以通过荧光团与金属纳米膜上的表面等离子体激元之间的相互作用来诱导。我们对光滑的金属纳米膜的研究应为利用其均匀的荧光增强特性进行生物成像铺平道路。
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