Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice.,Glia

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Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice.
Glia ( IF 5.4 ) Pub Date : 2020-03-16 , DOI: 10.1002/glia.23817
Sooyeon Yoo ₁ , David Cha ₁ , Soohyun Kim ₂ , Lizhi Jiang ₁ , Patrick Cooke ₁ , Mobolanie Adebesin ₁ , Andrew Wolfe ₃ , Ryan Riddle _{2,

4} , Susan Aja ₁ , Seth Blackshaw _{1,

5,

6,

7,

8}

Affiliation

Tanycytes are radial glial cells located in the mediobasal hypothalamus. Recent studies have proposed that tanycytes play an important role in hypothalamic control of energy homeostasis, although this has not been directly tested. Here, we report the phenotype of mice in which tanycytes of the arcuate nucleus and median eminence were conditionally ablated in adult mice. Although the cerebrospinal fluid‐hypothalamic barrier was rendered more permeable following tanycyte ablation, neither the blood‐hypothalamic barrier nor leptin‐induced pSTAT3 activation in hypothalamic parenchyma were affected. We observed a significant increase in visceral fat distribution accompanying insulin insensitivity in male mice, without significant effect on either body weight or food intake. A high‐fat diet tended to accelerate overall body weight gain in tanycyte‐ablated mice, but the development of visceral adiposity and insulin insensitivity was comparable to wildtype. Thermoneutral housing exacerbated fat accumulation and produced a shift away from fat oxidation in tanycyte‐ablated mice. These results clarify the extent to which tanycytes regulate energy balance, and demonstrate a role for tanycytes in regulating fat metabolism.

中文翻译：

弓状核和正中隆起中的 Tanycyte 消融通过增加雄性小鼠的体脂含量来增加肥胖易感性。

Tanycytes 是位于中底下丘脑的放射状胶质细胞。最近的研究表明，tanycytes 在能量稳态的下丘脑控制中起着重要作用，尽管这尚未得到直接测试。在这里，我们报告了成年小鼠有条件地消融弓状核和正中隆突的长核细胞的小鼠表型。尽管在tanycyte消融后脑脊液-下丘脑屏障变得更具渗透性，但血-下丘脑屏障和瘦素诱导的下丘脑实质中的pSTAT3激活均未受到影响。我们观察到雄性小鼠内脏脂肪分布显着增加，同时对胰岛素不敏感，对体重或食物摄入量没有显着影响。高脂肪饮食倾向于加速 tanycyte 消融小鼠的整体体重增加，但内脏肥胖和胰岛素不敏感的发展与野生型相当。在 tanycyte 消融的小鼠中，热中性住房加剧了脂肪积累并产生了远离脂肪氧化的转变。这些结果阐明了 tanycytes 调节能量平衡的程度，并证明了 tanycytes 在调节脂肪代谢中的作用。

更新日期：2020-03-16

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