• Plant oils are valuable commodities for food, feed, renewable industrial feedstocks and biofuels. To increase vegetable oil production, here we show that the nonspecific phospholipase C6 (NPC6) promotes seed oil production in the Brassicaceae seed oil species Arabidopsis, Camelina and oilseed rape.
• Overexpression of NPC6 increased seed oil content, seed weight and oil yield both in Arabidopsis and Camelina, whereas knockout of NPC6 decreased seed oil content and seed size. NPC6 is associated with the chloroplasts and microsomal membranes, and hydrolyzes phosphatidylcholine and galactolipids to produce diacylglycerol. Knockout and overexpression of NPC6 decreased and increased, respectively, the flux of fatty acids from phospholipids and galactolipids into triacylglycerol production.
• Candidate‐gene association study in oilseed rape indicates that only BnNPC6.C01 of the four homeologues NPC6s is associated with seed oil content and yield. Haplotypic analysis indicates that the BnNPC6.C01 favorable haplotype can increase both seed oil content and seed yield.
• These results indicate that NPC6 promotes membrane glycerolipid turnover to accumulate TAG production in oil seeds and that NPC6 has a great application potential for oil yield improvement.

中文翻译：

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非特异性磷脂酶C6增加了油菜花科植物中种子油的产量。

• 植物油是用于食品，饲料，可再生工业原料和生物燃料的有价值的商品。为了增加植物油的产量，我们在这里显示了非特异性磷脂酶C6（NPC6）促进了十字花科种子油物种拟南芥，山茶和油菜的种子油产量。
• NPC6的过表达增加了拟南芥和山茶中的种子油含量，种子重量和油产量，而敲除NPC6则降低了种子油含量和种子大小。NPC6与叶绿体和微粒体膜相关，并水解磷脂酰胆碱和半乳糖脂以产生二酰基甘油。NPC6的敲除和过表达分别降低和增加了从磷脂和半乳糖脂到三酰甘油生产的脂肪酸通量。
• 对油菜的候选基因关联研究表明，四种同源物NPC6中只有BnNPC6.C01与种子油含量和产量相关。单倍型分析表明，BnNPC6.C01有利的单倍型可以增加种子油含量和种子产量。
• 这些结果表明，NPC6促进膜甘油脂更新，以积累油料种子中的TAG产生，并且NPC6在提高油产量方面具有巨大的应用潜力。