当前位置：
X-MOL 学术
›
Plant Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Enhanced exudation of malate in the rhizosphere due to AtALMT1 overexpression in blackgram (Vigna mungo L.) confers increased aluminium tolerance.
Plant Biology ( IF 4.2 ) Pub Date : 2020-03-16 , DOI: 10.1111/plb.13112 B Saha 1 , D Swain 2 , P Borgohain 1 , G R Rout 2 , H Koyama 3 , S K Panda 1
中文翻译：
Blackgram（Vigna mungo L.）中AtALMT1的过度表达导致根际苹果酸分泌增加，从而提高了铝的耐受性。
更新日期：2020-03-16
Plant Biology ( IF 4.2 ) Pub Date : 2020-03-16 , DOI: 10.1111/plb.13112 B Saha 1 , D Swain 2 , P Borgohain 1 , G R Rout 2 , H Koyama 3 , S K Panda 1
Affiliation
|
- Worldwide, 50% of soil is acidic, which induces aluminium (Al) toxicity in plants, as the phyto‐availability of Al3+ increases in acidic soil. Plants responds to Al3+ toxicity by exuding organic acids into the rhizosphere. The organic acid responsible for Al3+ stress response varies from species to species, which in the case of blackgram (Vigna mungo L.) is citrate.
- In blackgram, an Arabidopsis malate transporter, At ALMT1, was overexpressed with the motive of inducing enhanced exudation of malate. Transgenics were generated using cotyledon node explants through Agrobacterium tumefaciens ‐mediated transformation. The putative transgenics were initially screened by At ALMT1‐specific genomic DNA PCR, followed by quantitative PCR. Two independent transgenic events were identified and functionally characterized in the T3 generation.
- The transgenic lines, Line 1 and 2, showed better root growth, relative water content and chlorophyll content under Al3+ stress. Both lines also accounted for less oxidative damage, due to reduced accumulation of ROS molecules. Photosynthetic efficiency, as measured in terms of Fv/Fm , NPQ and Y(II), increased when compared to the wild type (WT). Relative expression of genes (Vm STOP1, Vm ALS3, Vm MATE) responsible for Al3+ stress response in blackgram showed that overexpression of a malate transporter did not have any effect on their expression. Malate exudation increased whereas citrate exudation did not show any divergence from the WT. A pot stress assay found that the transgenics showed better adaptation to acidic soil.
- This report demonstrates that the overexpression of a malate transporter in a non‐malate exuding species improves adaptation to Al3+ toxicity in acidic soil without effecting its stress response mechanism.
中文翻译：
Blackgram（Vigna mungo L.）中AtALMT1的过度表达导致根际苹果酸分泌增加，从而提高了铝的耐受性。
- 在全球范围内，由于酸性土壤中Al 3+的植物利用率增加，因此50％的土壤是酸性的，从而在植物中引起铝（Al）毒性。植物通过向根际渗出有机酸来应对Al 3+毒性。负责Al 3+胁迫反应的有机酸因种而异，在黑豆gram（Vigna mungo L.）的情况下为柠檬酸盐。
- 在黑糖中，拟南芥苹果酸转运蛋白，在ALMT1，过表达以诱导苹果酸分泌增加。通过根癌农杆菌介导的转化，使用子叶节外植体产生转基因。首先通过At ALMT1特异性基因组DNA PCR筛选推定的转基因，然后进行定量PCR。在T 3世代中鉴定了两个独立的转基因事件并在功能上进行了表征。
- 在Al 3+胁迫下，转基因品系1和2表现出更好的根系生长，相对水含量和叶绿素含量。由于减少了ROS分子的积累，两条线也导致较少的氧化损伤。与野生型（WT）相比，以F v / F m ，NPQ和Y（II）衡量的光合效率有所提高。负责Al 3+的基因（Vm STOP1，Vm ALS3，Vm MATE）的相对表达黑图中的应激反应表明苹果酸转运蛋白的过表达对其表达没有任何影响。苹果酸分泌增加，而柠檬酸渗出与野生型无任何差异。盆栽试验发现转基因植物对酸性土壤的适应性更好。
- 该报告表明，苹果酸转运蛋白在非苹果酸分泌物种中的过表达提高了对酸性土壤中Al 3+毒性的适应性，而没有影响其胁迫响应机制。
京公网安备 11010802027423号