Novel nucleic acid detection strategies based on CRISPR-Cas systems: From construction to application.,Biotechnology and Bioengineering

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Novel nucleic acid detection strategies based on CRISPR-Cas systems: From construction to application.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-03-16 , DOI: 10.1002/bit.27334
Chu-Shu Zhu ₁ , Chuan-Yang Liu ₁ , Xin-Yuan Qiu ₁ , Si-Si Xie ₁ , Wen-Ying Li ₁ , Lingyun Zhu ₁ , Lv-Yun Zhu ₁

Affiliation

Beyond their widespread application as genome‐editing and regulatory tools, clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated (Cas) systems also play a critical role in nucleic acid detection due to their high sensitivity and specificity. Recently developed Cas family effectors have opened the door to the development of new strategies for detecting different types of nucleic acids for a variety of purposes. Precise and efficient nucleic acid detection using CRISPR‐Cas systems has the potential to advance both basic and applied biological research. In this review, we summarize the CRISPR‐Cas systems used for the recognition and detection of specific nucleic acids for different purposes, including the detection of genomic DNA, nongenomic DNA, RNA, and pathogenic microbe genomes. Current challenges and further applications of CRISPR‐based detection methods will be discussed according to the most recent developments.

中文翻译：

基于 CRISPR-Cas 系统的新型核酸检测策略：从构建到应用。

除了作为基因组编辑和调控工具的广泛应用之外，成簇的规则间隔短回文重复序列 (CRISPR)-CRISPR 相关 (Cas) 系统由于其高灵敏度和特异性，在核酸检测中也发挥着关键作用。最近开发的 Cas 家族效应器为开发用于检测各种用途的不同类型核酸的新策略打开了大门。使用 CRISPR-Cas 系统进行精确高效的核酸检测有可能促进基础和应用生物学研究。在这篇综述中，我们总结了用于识别和检测不同目的特定核酸的 CRISPR-Cas 系统，包括检测基因组 DNA、非基因组 DNA、RNA 和病原微生物基因组。

更新日期：2020-03-16

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