Engineering d-Lactate Dehydrogenase to Favor an Non-natural Cofactor Nicotinamide Cytosine Dinucleotide.,ChemBioChem

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Engineering d-Lactate Dehydrogenase to Favor an Non-natural Cofactor Nicotinamide Cytosine Dinucleotide.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-03-16 , DOI: 10.1002/cbic.201900766
Yuxue Liu ₁ , Qing Li ₁ , Lei Wang ₁ , Xiaojia Guo ₁ , Junting Wang ₁ , Qian Wang _{1,

2} , Zongbao K Zhao _{1,

2,

3}

Affiliation

Bioorthogonal metabolism ? Synthetic NAD analogue NCD has already been used to create bioorthogonal redox systems. We have engineered L. helveticus‐derived NAD‐dependent d ‐lactate dehydrogenase (Lh DLDH) to favor NCD by semirational design. Libraries were constructed from Lh DLDH‐V152R led to mutants with up to 940‐fold increased preference for NCD.

中文翻译：

工程d-乳酸脱氢酶，以偏爱非天然辅因子烟酰胺胞嘧啶二核苷酸。

生物正交代谢？合成的NAD模拟物NCD已用于创建生物正交氧化还原系统。我们设计的瑞士乳来源的NAD依赖性d -乳酸脱氢酶（Lh的DLDH）由semirational设计有利于NCD。利用Lh DLDH-V152R构建的库导致突变体对NCD的偏好提高了940倍。

更新日期：2020-03-16

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