The influence of the addition of granular activated carbon (GAC) on the anaerobic treatment of wastewater with a mixture of ethanol and 1-ethoxy-2-propanol (E2P), which are typical pollutants from the flexographic/printing industry, was evaluated. For this purpose, the reactor performance and microbial community in two continuous stirred tank reactors (CSTRs), i.e. control and GAC supplemented, were assessed. Both reactors, seeded with anaerobic granular biomass, showed similar global performance (RE > 93%), except after organic load shocks and after E2P addition. GAC supplementation substantially enhanced the response of the biological system to organic load shocks and reduced the acclimation period for E2P degradation. This improvement was found to be related to the effect of GAC on the microbial community which, in turn, is related to an increase in the degradation rate of intermediates (acetone and isopropanol). The microbial community was analysed through denaturing gradient gel electrophoresis and high-throughput sequencing. Both methods showed an evolution from the inoculum to the final time point and considerable enrichment in microorganisms involved in direct interspecies electron transfer, such as Geobacter species in the final biomass samples, especially in the biomass attached to the GAC.

中文翻译：

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在连续搅拌釜反应器中，活性炭对处理溶剂污染物混合物的性能和微生物群落的影响

评估了添加颗粒状活性炭（GAC）对乙醇和1-乙氧基-2-丙醇（E2P）的混合物进行厌氧处理废水的影响，乙醇和苯乙氧基-2-丙醇是柔性版/印刷行业的典型污染物。为了这个目的，两个连续搅拌釜反应器（CSTR）的反应器的性能和微生物群落，即对照和补充GAC，进行评估。接种厌氧颗粒生物质的两个反应堆均表现出相似的整体性能（RE> 93％），除了有机负荷冲击之后和添加E2P之后。GAC的补充大大增强了生物系统对有机负荷冲击的响应，并缩短了E2P降解的适应期。发现这种改善与GAC对微生物群落的影响有关，而GAC对微生物群落的影响又与中间体（丙酮和异丙醇）降解速率的增加有关。通过变性梯度凝胶电泳和高通量测序分析了微生物群落。两种方法均显示出从接种物到最终时间点的演变，并且大量富集了直接种间电子转移所涉及的微生物，最终生物质样品中的地细菌种类，尤其是附着在GAC上的生物质中。