High-throughput protein screening is a critical technique for dissecting and designing protein function. Libraries for these assays can be created through a number of means, including targeted or random mutagenesis of a template protein sequence or direct DNA synthesis. However, mutagenic library construction methods often yield vastly more nonfunctional than functional variants and, despite advances in large-scale DNA synthesis, individual synthesis of each desired DNA template is often prohibitively expensive. Consequently, many protein-screening libraries rely on the use of degenerate codons (DCs), mixtures of DNA bases incorporated at specific positions during DNA synthesis, to generate highly diverse protein-variant pools from only a few low-cost synthesis reactions. However, selecting DCs for sets of sequences that covary at multiple positions dramatically increases the difficulty of designing a DC library and leads to the creation of many undesired variants that can quickly outstrip screening capacity.

中文翻译：

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DeCoDe：用于完整蛋白质编码DNA库的简并密码子设计。

高通量蛋白质筛选是剖析和设计蛋白质功能的关键技术。可以通过多种方式创建用于这些测定的文库，包括靶向或随机诱变模板蛋白质序列或直接进行DNA合成。然而，诱变文库构建方法通常比功能性变体产生更多的非功能性，并且尽管大规模DNA合成取得了进展，但每个所需DNA模板的单独合成通常非常昂贵。因此，许多蛋白质筛选库都依赖于简并密码子（DC）的使用，简并密码子（DNA）在DNA合成过程中的特定位置掺入DNA的混合物，仅通过少量低成本的合成反应即可产生高度多样化的蛋白质变异库。然而，