当前位置：
X-MOL 学术
›
Nat. Methods
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
3D ATAC-PALM: super-resolution imaging of the accessible genome.
Nature Methods ( IF 36.1 ) Pub Date : 2020-03-16 , DOI: 10.1038/s41592-020-0775-2 Liangqi Xie 1, 2, 3 , Peng Dong 1 , Xingqi Chen 4, 5 , Tsung-Han S Hsieh 2, 3 , Sambashiva Banala 1 , Margherita De Marzio 6 , Brian P English 1 , Yifeng Qi 6 , Seol Kyoung Jung 7 , Kyong-Rim Kieffer-Kwon 7 , Wesley R Legant 1 , Anders S Hansen 2 , Anton Schulmann 1 , Rafael Casellas 7 , Bin Zhang 6 , Eric Betzig 1 , Luke D Lavis 1 , Howard Y Chang 4 , Robert Tjian 2, 3 , Zhe Liu 1
Nature Methods ( IF 36.1 ) Pub Date : 2020-03-16 , DOI: 10.1038/s41592-020-0775-2 Liangqi Xie 1, 2, 3 , Peng Dong 1 , Xingqi Chen 4, 5 , Tsung-Han S Hsieh 2, 3 , Sambashiva Banala 1 , Margherita De Marzio 6 , Brian P English 1 , Yifeng Qi 6 , Seol Kyoung Jung 7 , Kyong-Rim Kieffer-Kwon 7 , Wesley R Legant 1 , Anders S Hansen 2 , Anton Schulmann 1 , Rafael Casellas 7 , Bin Zhang 6 , Eric Betzig 1 , Luke D Lavis 1 , Howard Y Chang 4 , Robert Tjian 2, 3 , Zhe Liu 1
Affiliation
|
To image the accessible genome at nanometer scale in situ, we developed three-dimensional assay for transposase-accessible chromatin-photoactivated localization microscopy (3D ATAC-PALM) that integrates an assay for transposase-accessible chromatin with visualization, PALM super-resolution imaging and lattice light-sheet microscopy. Multiplexed with oligopaint DNA-fluorescence in situ hybridization (FISH), RNA-FISH and protein fluorescence, 3D ATAC-PALM connected microscopy and genomic data, revealing spatially segregated accessible chromatin domains (ACDs) that enclose active chromatin and transcribed genes. Using these methods to analyze genetically perturbed cells, we demonstrated that genome architectural protein CTCF prevents excessive clustering of accessible chromatin and decompacts ACDs. These results highlight 3D ATAC-PALM as a useful tool to probe the structure and organizing mechanism of the genome.
中文翻译：
3D ATAC-PALM：可访问基因组的超分辨率成像。
为了在纳米尺度上原位成像可访问的基因组，我们开发了转座酶可访问染色质光激活定位显微镜 (3D ATAC-PALM) 的三维测定法，该方法将转座酶可访问染色质的测定与可视化、PALM 超分辨率成像和晶格光片显微镜。多路复用 oligopaint DNA 荧光原位杂交 (FISH)、RNA-FISH 和蛋白质荧光，3D ATAC-PALM 连接显微镜和基因组数据，揭示了空间分离的可接近染色质结构域 (ACD)，其中包含活性染色质和转录基因。使用这些方法分析遗传扰动的细胞，我们证明了基因组结构蛋白 CTCF 可防止可接近染色质的过度聚集并分解 ACD。
更新日期：2020-03-16
中文翻译：
3D ATAC-PALM：可访问基因组的超分辨率成像。
为了在纳米尺度上原位成像可访问的基因组，我们开发了转座酶可访问染色质光激活定位显微镜 (3D ATAC-PALM) 的三维测定法，该方法将转座酶可访问染色质的测定与可视化、PALM 超分辨率成像和晶格光片显微镜。多路复用 oligopaint DNA 荧光原位杂交 (FISH)、RNA-FISH 和蛋白质荧光，3D ATAC-PALM 连接显微镜和基因组数据，揭示了空间分离的可接近染色质结构域 (ACD)，其中包含活性染色质和转录基因。使用这些方法分析遗传扰动的细胞，我们证明了基因组结构蛋白 CTCF 可防止可接近染色质的过度聚集并分解 ACD。
京公网安备 11010802027423号