Polysaccharides derived from microorganisms have received considerable attention in designing hydrogel materials. However, most microbial polysaccharide-constructed hydrogels evaluated in preclinical trials are not favorable candidates for biomedical applications owing to concerns regarding poor mechanical strength and complicated fabrication process. Herein, we describe a new polysaccharide hydrogel scaffold containing salecan together with gellan gum network as the polymeric matrix. Properly controlling the physical and chemical properties including swelling, water release, thermal stability, viscoelasticity and morphology of the resulting gel are easily achieved by simply changing the salecan/gellan gum ratios. Notably, these salecan/gellan gum scaffolds friendly support cell survival and proliferation. More significantly, we have systematically evaluated these developed hydrogels for the biocompatible experiments in vitro and in vivo and results indicated the products are non-toxic. Taken together, such hydrogels derived from microbial polysaccharides and readily synthesized through a one-step mixing protocol have translational potentials in the clinic serving as cell devices for tissue engineering.

在设计水凝胶材料时，源自微生物的多糖受到了相当大的关注。但是，由于担心机械强度差和制造工艺复杂，在临床前试验中评估的大​​多数微生物多糖构建的水凝胶都不适合用于生物医学应用。在本文中，我们描述了一种新的多糖水凝胶支架，其包含salecan以及结冷胶网络作为聚合物基质。只需更改salecan / gellan胶的比例，即可轻松控制所得凝胶的物理和化学性质，包括溶胀，释水，热稳定性，粘弹性和形态。值得注意的是，这些salcan /吉兰糖胶支架友好地支持细胞存活和增殖。更重要的是在体外和体内，结果表明该产品无毒。综上所述，这种源自微生物多糖的水凝胶很容易通过一步混合法合成，在临床上具有作为组织工程细胞装置的翻译潜力。