The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord,Biomaterials

当前位置： X-MOL 学术 › Biomaterials › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord
Biomaterials ( IF 12.8 ) Pub Date : 2020-03-16 , DOI: 10.1016/j.biomaterials.2020.119978
Xiaowei Li ₁ , Chi Zhang ₂ , Agnes E Haggerty ₃ , Jerry Yan ₄ , Michael Lan ₄ , Michelle Seu ₅ , Mingyu Yang ₁ , Megan M Marlow ₃ , Inés Maldonado-Lasunción ₆ , Brian Cho ₅ , Zhengbing Zhou ₁ , Long Chen ₁ , Russell Martin ₁ , Yohshiro Nitobe ₇ , Kentaro Yamane ₈ , Hua You ₉ , Sashank Reddy ₁₀ , Da-Ping Quan ₁₁ , Martin Oudega ₁₂ , Hai-Quan Mao ₁₃

Affiliation

Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA.
Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; School of Chemistry, Sun Yat-Sen University, Guangzhou, Guangdong 510275, PR China.
The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA.
Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA; Department of Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands; Shirley Ryan AbilityLab, Chicago, IL 60611, USA; Department of Physical Therapy and Human Movements Sciences, Chicago, IL 60611, USA; Department of Physiology Northwestern University, Chicago, IL 60611, USA.
The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA; Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, 036-8562, Japan.
The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA; Department of Orthopedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science, Kitaku, Okayama, 700-8558, Japan.
Affiliated Cancer Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510095, PR China.
Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
School of Chemistry, Sun Yat-Sen University, Guangzhou, Guangdong 510275, PR China.
Shirley Ryan AbilityLab, Chicago, IL 60611, USA; Department of Physical Therapy and Human Movements Sciences, Chicago, IL 60611, USA; Department of Physiology Northwestern University, Chicago, IL 60611, USA; Affiliated Cancer Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510095, PR China; Edward Hines Jr. VA Hospital, Hines IL, 60141, USA.
Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.

An injury to the spinal cord causes long-lasting loss of nervous tissue because endogenous nervous tissue repair and regeneration at the site of injury is limited. We engineered an injectable nanofiber-hydrogel composite (NHC) with interfacial bonding to provide mechanical strength and porosity and examined its effect on repair and neural tissue regeneration in an adult rat model of spinal cord contusion. At 28 days after treatment with NHC, the width of the contused spinal cord segment was 2-fold larger than in controls. With NHC treatment, tissue in the injury had a 2-fold higher M2/M1 macrophage ratio, 5-fold higher blood vessel density, 2.6-fold higher immature neuron presence, 2.4-fold higher axon density, and a similar glial scar presence compared with controls. However, the spared nervous tissue volume in the contused segment and hind limb function was similar between groups. Our findings indicated that NHC provided mechanical support to the contused spinal cord and supported pro-regenerative macrophage polarization, angiogenesis, axon growth, and neurogenesis in the injured tissue without any exogenous factors or cells. These results motivate further optimization NHC and delivery protocol to fully translate the potential of the unique properties of NHC for treating spinal cord injury.

中文翻译：

纳米纤维-水凝胶复合材料对挫伤脊髓神经组织修复和再生的影响

脊髓损伤会导致神经组织的长期损失，因为损伤部位的内源性神经组织修复和再生是有限的。我们设计了一种具有界面结合的可注射纳米纤维-水凝胶复合材料 (NHC)，以提供机械强度和孔隙率，并在成年大鼠脊髓挫伤模型中检查其对修复和神经组织再生的影响。在用 NHC 治疗后 28 天，挫伤脊髓节段的宽度比对照组大 2 倍。与 NHC 治疗相比，损伤组织的 M2/M1 巨噬细胞比率高出 2 倍，血管密度高出 5 倍，未成熟神经元存在高出 2.6 倍，轴突密度高出 2.4 倍，并且与类似的胶质瘢痕存在相比与控件。然而，各组间挫伤节段的剩余神经组织体积和后肢功能相似。我们的研究结果表明，NHC 为受挫伤的脊髓提供机械支持，并支持损伤组织中的促再生巨噬细胞极化、血管生成、轴突生长和神经发生。任何外源性因素或细胞。这些结果促使进一步优化 NHC 和递送方案，以充分发挥 NHC 治疗脊髓损伤的独特特性的潜力。

更新日期：2020-03-16

点击分享查看原文

点击收藏

阅读更多本刊最新论文