The binding of an anti-diabetic drug rosiglitazone (RG) with calf-thymus DNA (CT-DNA) in physiological buffer (pH 7.4) has been investigated using various spectral techniques such as UV–Vis, fluorescence, ¹H NMR and circular dichroism (CD) coupled with viscosity measurement and molecular docking studies. The binding of RG with CT-DNA results in small hypochromism without any change in absorption maximum and fluorescence quenching with hardly any shifts in emission maximum suggesting groove binding mode of interaction. The binding constant is found to be 4.2 × 10² M⁻¹ at 298 K. Thermodynamic analysis reveal that the binding is spontaneous and H-bonding and van der Waals forces play predominant role in the binding of RG with CT-DNA. Competitive interaction between RG and ethidium bromide with CT-DNA, viscosity measurements, KI quenching, ¹H NMR and CD studies substantiate the prosed mode of binding. Voltammetric investigations suggest that the electro-reduction of RG is an adsorption controlled process and shift of reduction peak to more negative potential, with a binding constant of 3.4 × 10³ M⁻¹, validates the groove binding mode of interaction between RG and CT-DNA. Molecular docking reveals that RG binds in the minor groove of DNA and the dominating interaction forces are H-bonding and hydrophobic interactions.

使用多种光谱技术，例如紫外-可见，荧光，¹ H NMR和圆二色性，研究了抗糖尿病药罗格列酮（RG）与小牛胸腺DNA（CT-DNA）在生理缓冲液（pH 7.4）中的结合。（CD）以及粘度测量和分子对接研究。RG与CT-DNA的结合导致小的变色现象，吸收最大值没有任何变化，并且荧光猝灭几乎没有发射最大值的变化，表明了相互作用的凹槽结合模式。发现结合常数为4.2×10 ² M ^-1在298 K时的热力学分析表明，该结合是自发的，H键和范德华力在RG与CT-DNA的结合中起主要作用。RG和溴化乙锭与CT-DNA的竞争性相互作用，粘度测量，KI猝灭，¹ H NMR和CD研究证实了所认为的结合方式。伏安研究表明，RG的电还原是一个受吸附控制的过程，还原峰向负电位移动，结合常数为3.4×10 ³ M ^-1，验证了RG与CT-的相互作用的沟槽结合模式。脱氧核糖核酸。分子对接揭示了RG 在DNA的小沟中结合，主要的相互作用力是H键和疏水相互作用。