Short-chain fatty acids (SCFAs) alteration have been reported in irritable bowel syndrome (IBS), but the results are conflicting. Our study aims to explore the alteration of SCFAs in patients with diarrhea-predominant IBS (IBS-D) and their potential role in the occurrence and development of IBS. We recruited patients with IBS-D defined by Rome IV criteria and age-and-gender matched healthy controls (HCs). A headspace solid-phase microextraction gas chromatography–mass spectrometric (HS-SPME-GC-MS) method was developed for the analysis of acetic, propionic and butyric acid in feces and serum. Compared with HCs, the levels of the serum propionate (2.957 ± 0.157 vs 2.843 ± 0.098 mmol/L, P = 0.012) and butyrate (2.798 ± 0.126 vs 2.697 ± 0.077 mmol/L, P = 0.012) were significantly higher in IBS-D group. No significant differences were found among two groups with regard to the concentration of fecal acetate (4.953 ± 1.065 vs 4.774 ± 1.465 mg/g, P = 0.679), propionate (6.342 ± 1.005 vs 6.282 ± 1.077 mg/g, P = 0.868) and butyrate (2.984 ± 0.512 vs 3.071 ± 0.447 mg/g, P = 0.607). Metabolites of gut microbiota, the propionic and butyric acid, are increased in patients with IBS-D in serum but not in feces. It suggests that propionic and butyric acid might be associated with the occurrence and development of IBS.

中文翻译：

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腹泻型肠易激综合征患者血清中丙酸和丁酸升高，但粪便中丙酸和丁酸升高

在肠易激综合症（IBS）中已报道了短链脂肪酸（SCFA）的改变，但结果相矛盾。我们的研究旨在探讨腹泻型IBS（IBS-D）患者中SCFA的变化及其在IBS发生和发展中的潜在作用。我们招募了具有罗马IV标准和年龄和性别匹配的健康对照（HCs）定义的IBS-D患者。建立了顶空固相微萃取气相色谱-质谱（HS-SPME-GC-MS）方法，用于分析粪便和血清中的乙酸，丙酸和丁酸。与HCs相比，IBS-血清中丙酸（2.957±0.157 vs 2.843±0.098 mmol / L，P = 0.012）和丁酸（2.798±0.126 vs 2.697±0.077 mmol / L，P = 0.012）的水平明显升高。 D组。两组之间的粪便乙酸盐（4.953±1.065 vs 4.774±1.465 mg / g，P = 0.679），丙酸（6.342±1.005 vs 6.282±1.077 mg / g，P = 0.868）的浓度之间无显着差异。和丁酸盐（2.984±0.512 vs 3.071±0.447 mg / g，P = 0.607）。IBS-D患者血清中肠道菌群，丙酸和丁酸的代谢产物增加，但粪便中的代谢产物没有增加。这表明丙酸和丁酸可能与IBS的发生和发展有关。血清中IBS-D患者的血脂升高，但粪便中的血脂升高。这表明丙酸和丁酸可能与IBS的发生和发展有关。血清中IBS-D患者的血脂升高，但粪便中的血脂升高。这表明丙酸和丁酸可能与IBS的发生和发展有关。