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Direct Current Stimulation for Improved Osteogenesis of MC3T3 Cells Using Mineralized Conductive Polyaniline
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-15 , DOI: 10.1021/acsbiomaterials.9b01821 Quanxin Wang 1, 2 , Mingzhen Wu 1 , Xiaoyang Xu 1 , Chunmei Ding 1 , Jianbin Luo 2 , Jianshu Li 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-15 , DOI: 10.1021/acsbiomaterials.9b01821 Quanxin Wang 1, 2 , Mingzhen Wu 1 , Xiaoyang Xu 1 , Chunmei Ding 1 , Jianbin Luo 2 , Jianshu Li 1
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Hydroxyapatites (HAPs) are usually coated on the surface of an implant to improve the osseointegration with defect bone tissue. Besides, conducting polymers have the advantages of good conductivity, reasonable biocompatibility, and easy of modification, which endow them applicable to electrical stimulation therapy. However, it still remains a great challenge to fabricate hybrid coating combing HAP with conducting polymer on implant surface efficiently. In this work, phytic acid-doped polyaniline (PANI) were successfully synthesized on medical titanium (Ti) sheets. By virtue of the abundant anodic phosphoric groups of phytic acid, HAP nanocrystals were biomineralized on PANI. The PANI-HAP hybrid layer exhibits good cell compatibility with MC3T3 cells. More importantly, HAP nanocrystals and PANI operate synergistically on cell proliferation and osteogenesis through electrical stimulation. Alkaline phosphatase activity and extracellular calcium contents of cells on PANI-HAP display 3-fold and 2.6-fold increases, compared with bare Ti sheets, respectively. The valid integration of mineralization and electrical stimulation in this work renders an efficient strategy for implant coating, which might have potential applications in bone-related defects.
中文翻译：
直流电刺激使用矿化的导电聚苯胺改善MC3T3细胞的成骨作用
羟基磷灰石（HAP）通常涂覆在植入物的表面上，以改善与缺损骨组织的骨整合。此外，导电聚合物具有导电性好，生物相容性好，易于改性的优点，使其适用于电刺激疗法。然而，在混合材料涂层中将HAP与导电聚合物有效地结合在植入物表面上仍然是一个巨大的挑战。在这项工作中，成功地在医用钛（Ti）片上合成了植酸掺杂的聚苯胺（PANI）。由于植酸具有丰富的阳极磷酸基团，HAP纳米晶体在PANI上被生物矿化。PANI-HAP杂化层表现出与MC3T3细胞的良好细胞相容性。更重要的是，HAP纳米晶体和PANI通过电刺激协同作用促进细胞增殖和成骨。与裸钛板相比，PANI-HAP上的细胞碱性磷酸酶活性和细胞外钙含量分别增加了3倍和2.6倍。在这项工作中矿化和电刺激的有效结合为植入物涂层提供了有效的策略，这可能在与骨相关的缺陷中具有潜在的应用。
更新日期：2020-03-15
中文翻译：
直流电刺激使用矿化的导电聚苯胺改善MC3T3细胞的成骨作用
羟基磷灰石（HAP）通常涂覆在植入物的表面上，以改善与缺损骨组织的骨整合。此外，导电聚合物具有导电性好，生物相容性好，易于改性的优点，使其适用于电刺激疗法。然而，在混合材料涂层中将HAP与导电聚合物有效地结合在植入物表面上仍然是一个巨大的挑战。在这项工作中，成功地在医用钛（Ti）片上合成了植酸掺杂的聚苯胺（PANI）。由于植酸具有丰富的阳极磷酸基团，HAP纳米晶体在PANI上被生物矿化。PANI-HAP杂化层表现出与MC3T3细胞的良好细胞相容性。更重要的是，HAP纳米晶体和PANI通过电刺激协同作用促进细胞增殖和成骨。与裸钛板相比，PANI-HAP上的细胞碱性磷酸酶活性和细胞外钙含量分别增加了3倍和2.6倍。在这项工作中矿化和电刺激的有效结合为植入物涂层提供了有效的策略，这可能在与骨相关的缺陷中具有潜在的应用。
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