Diabetic patients display increased risk of periodontitis and failure in bone augmentation procedures. Mesenchymal stem cells (MSCs) and platelet‐rich plasma (PRP) represent a relevant advantage in tissue repair process and regenerative medicine. We isolated MSCs from Bichat's buccal fat pad (BFP) and measured the effects of glucose and PRP on cell number and osteogenic differentiation potential. Cells were cultured in the presence of 5.5‐mM glucose (low glucose [LG]) or 25‐mM glucose (high glucose [HG]). BFP–MSC number was significantly lower when cells were cultured in HG compared with those in LG. Following osteogenic differentiation procedures, calcium accumulation, alkaline phosphatase activity, and expression of osteogenic markers were significantly lower in HG compared with LG. Exposure of BFP–MSC to PRP significantly increased cell number and osteogenic differentiation potential, reaching comparable levels in LG and in HG. Thus, high‐glucose concentrations impair BFP–MSC growth and osteogenic differentiation. However, these detrimental effects are largely counteracted by PRP.

中文翻译：

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富含血小板的血浆可抵消高糖浓度对Bichat脂肪垫的间充质干细胞的有害作用。

糖尿病患者出现牙周炎和骨骼增强手术失败的风险增加。间充质干细胞（MSC）和富含血小板的血浆（PRP）在组织修复过程和再生医学中具有相关优势。我们从Bichat的颊脂肪垫（BFP）中分离了MSC，并测量了葡萄糖和PRP对细胞数量和成骨分化潜能的影响。在存在5.5-mM葡萄糖（低葡萄糖[LG]）或25-mM葡萄糖（高葡萄糖[HG]）的情况下培养细胞。在HG中培养的细胞中，与LG中的细胞相比，BFP–MSC的数量明显更低。经过成骨分化程序后，与LG相比，HG中的钙积累，碱性磷酸酶活性和成骨标记物的表达明显降低。BFP–MSC与PRP的接触显着增加了细胞数量和成骨分化潜能，在LG和HG中达到了相当的水平。因此，高血糖会损害BFP–MSC的生长和成骨分化。但是，这些有害影响在很大程度上由PRP抵消。