Pancreatic cancer has a dismal prognosis and to date there are no targeted therapies for this malignancy. Using shotgun proteomics, the mRNA binding protein cold shock domain containing E1 (CSDE1), also called upstream-of-N-Ras, is detected in pancreatic cancer cell lines but not in normal pancreatic epithelial cells. The expression of CSDE1 in pancreatic cancer cells is confirmed by Western blotting and immunohistochemistry of human pancreatic tumors. In vitro functional assays show that siRNA downregulation of CSDE1 or gene knockout using CRISPR-Cas9 significantly reduce the invasiveness of pancreatic cancer cells. Together, this study reveals that CSDE1 is overexpressed in pancreatic cancer and is a potential therapeutic target to inhibit pancreatic cancer cell invasion.

中文翻译：

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含有E1（CSDE1）蛋白的冷激域过度表达，可以靶向抑制胰腺癌细胞的侵袭。

胰腺癌的预后不良，迄今为止，尚无针对这种恶性肿瘤的靶向疗法。使用shot弹枪蛋白质组学，在胰腺癌细胞系中检测到含有E1的mRNA结合蛋白冷休克结构域（CSDE1），也称为N-Ras上游，但在正常胰腺上皮细胞中未检测到。通过蛋白质印迹和人类胰腺肿瘤的免疫组织化学证实了CSDE1在胰腺癌细胞中的表达。体外功能分析表明，使用CRISPR-Cas9的CSDE1 siRNA下调或基因敲除显着降低了胰腺癌细胞的侵袭性。总之，这项研究表明CSDE1在胰腺癌中过表达，并且是抑制胰腺癌细胞入侵的潜在治疗靶标。