Epothilone is a macrolide secondary metabolite which has the same anticancer effect as paclitaxel. Based on a series of single‐factor experiments, four factors, temperature, initial pH, rotation speed, and inoculum quantity, which have the greatest influence on yield, were determined. Four factors were designed and orthogonal experiments were carried out to optimize the fermentation conditions. Finally, the best experimental conditions were obtained as follows: 250 ml flapper triangular flask was used. The yield of epothilone B was 39.76 mg/L at 30℃, initial pH = 7.4, rotating speed 200 r/min, inoculation amount 10%, liquid loading amount 50 ml/250 ml, fermentation time 6 days and seed age 60 hr.

中文翻译：

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优化生产埃坡霉素B的发酵条件

埃坡霉素是一种大环内酯类次生代谢产物，具有与紫杉醇相同的抗癌作用。在一系列单因素实验的基础上，确定了对产量影响最大的四个因素，即温度，初始pH，转速和接种量。设计了四个因素，并进行正交试验以优化发酵条件。最后，最佳实验条件如下：使用250 ml挡板三角烧瓶。在30℃，初始pH = 7.4，转速200 r / min，接种量10％，液体装载量50 ml / 250 ml，发酵时间6天和种子龄60小时的条件下，埃坡霉素B的产量为39.76 mg / L。