• Legumes interact with a wide range of microbes in their root systems, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so‐called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in the root epidermis.
• By combining gene expression analysis, mutant phenotypic screening and analysis of nuclear calcium elevations, we demonstrate that recognition of an endophytic Fusarium solani strain K (FsK) in model legumes is initiated via perception of chitooligosaccharidic molecules and is, at least partially, CSSP‐dependent.
• FsK induced the expression of Lysin‐motif receptors for chitin‐based molecules, CSSP members and CSSP‐dependent genes in Lotus japonicus. In LysM and CSSP mutant/RNAi lines, root penetration and fungal intraradical progression was either stimulated or limited, whereas FsK exudates triggered CSSP‐dependent nuclear calcium spiking, in epidermal cells of Medicago truncatula root organ cultures.
• Our results corroborate CSSP being involved in the perception of signals from other microbes beyond the restricted group of symbiotic interactions sensu stricto.

中文翻译：

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内生镰刀菌-豆科植物的关联部分取决于共同的共生信号通路

• 豆类与根系中的多种微生物相互作用，范围从有益的共生菌到病原体。共生的根瘤菌和丛枝菌根性肾小球菌触发了一种所谓的常见共生信号通路（CSSP），包括诱导根表皮中的钙核峰值。
• 通过结合基因表达分析，突变体表型筛选和核钙升高分析，我们证明了对模型豆科植物内生镰刀菌枯萎病菌株K（FsK）的识别是通过感知壳寡糖分子而引发的，并且至少部分是CSSP依赖性的。
• FSK诱导的细胞溶素基序受体基于壳多糖分子，CSSP成员和在CSSP依赖性基因的表达百脉根。在LysM和CSSP突变体/ RNAi品系中，在Medi藜苜蓿根器官培养物中的表皮细胞中，根部渗透和真菌的根际进程受到刺激或受到限制，而FsK渗出物触发了CSSP依赖性核钙的掺入。
• 我们的结果证实CSSP被卷入的从超出共生相互作用的受限组其他微生物的信号的感知狭义。