Iron deficiency chlorosis is an important abiotic stress affecting groundnut production worldwide in calcareous and alkaline soils with a pH of 7.5–8.5. To identify genomic regions controlling iron deficiency chlorosis resistance in groundnut, the recombinant inbred line population from the cross TAG 24 × ICGV 86031 was evaluated for associated traits like visual chlorosis rating and SPAD chlorophyll meter reading across three crop growth stages for two consecutive years. Thirty-two QTLs were identified for visual chlorosis rating (3.9%–31.8% phenotypic variance explained [PVE]) and SPAD chlorophyll meter reading [3.8%–11% PVE] across three stages over 2 years. This is the first report of identification of QTLs for iron deficiency chlorosis resistance- associated traits in groundnut. Three major QTLs (>10% PVE) were identified at severe stage, while majority of other QTLs were having small effects. Interestingly, two major QTLs for visual chlorosis rating at 60 days (2013) and 90 days (2014) were located at same position on LG AhXIII. The identified QTLs/markers after validation across diverse genetic material could be used in genomics-assisted breeding.

中文翻译：

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花生(Arachis hypogaea)缺铁性萎黄病抗性相关数量性状位点的鉴定

缺铁性萎黄病是一种重要的非生物胁迫，在 pH 值为 7.5-8.5 的钙质和碱性土壤中影响全球花生生产。为了确定控制花生缺铁性黄化抗性的基因组区域，连续两年评估了来自交叉 TAG 24 × ICGV 86031 的重组自交系群体的相关性状，如视觉黄化等级和 SPAD 叶绿素计读数跨越三个作物生长阶段。在 2 年的三个阶段中，确定了 32 个 QTL，用于视觉萎黄症等级（3.9%–31.8% 表型变异解释 [PVE]）和 SPAD 叶绿素仪表读数 [3.8%–11% PVE]。这是首次鉴定花生缺铁性萎黄病相关性状的QTL。在严重阶段鉴定了三个主要 QTL（>10% PVE），而大多数其他 QTL 的影响很小。有趣的是，在 60 天（2013 年）和 90 天（2014 年）时视觉萎黄病评级的两个主要 QTL 位于 LG AhXIII 上的相同位置。在对不同遗传材料进行验证后确定的 QTL/标记可用于基因组学辅助育种。