We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration. The 3D porous‐structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis. Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold. The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior. The degradation test demonstrated that the material is slowly degraded, while the addition of nHA slightly influences the degradation of the scaffolds. Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content. On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds. Altogether, our results allow us to conclude that these nanobiocomposites are promising candidates to induce bone tissue regeneration.

中文翻译：

---

基于天然聚电解质的纳米生物复合材料用于骨再生。

我们开发了一种基于壳聚糖和羧甲基纤维素的复合水凝胶，以纳米羟基磷灰石 (nHA) 作为填充剂（范围为 0.5% 至 5%），通过超声波方法用于骨再生。通过扫描电子显微镜和显微断层扫描分析证实了生物复合支架的 3D 多孔结构。红外分析未显示复合材料的有机成分与支架中的 nHA 之间的特定相互作用。通过溶胀和机械测试研究了基质的水凝胶特性，表明支架表现出良好的机械性能。降解试验表明材料降解缓慢，而 nHA 的加入对支架的降解有轻微影响。用骨髓间充质祖细胞 (BMPC) 进行的生物相容性研究表明，细胞增殖和碱性磷酸酶活性随基质 nHA 含量而增加。另一方面，没有观察到细胞毒性作用RAW264.7细胞接种在支架上。总之，我们的结果使我们能够得出结论，这些纳米生物复合材料是诱导骨组织再生的有希望的候选材料。