Studies of the toxicity of poly‐ and perfluoroalkyl substances (PFAS) on amphibians, especially after metamorphosis, are limited. We examined effects of dermal PFAS exposure (30 d) on survival and growth of juvenile American toads (Anaxyrus americanus), eastern tiger salamanders (Ambystoma tigrinum), and northern leopard frogs (Rana pipiens). Chemicals included perfluorooctanoic acid, perfluorooctane sulfonate (PFOS), perfluorohexane sulfonate (PFHxS), and 6:2 fluorotelomer sulfonate (6:2 FTS) at 0, 80, 800, or 8000 ppb on a moss dry weight basis. Exposure to PFAS influenced final snout–vent length (SVL) and scaled mass index (SMI), a measure of relative body condition. Observed effects depended on species and chemical, but not concentration. Anurans exposed to PFOS, PFHxS (frogs only), and 6:2 FTS demonstrated reduced SVL versus controls, whereas salamanders exposed to 6:2 FTS showed increased SVL. Frogs exposed to PFHxS and 6:2 FTS and toads exposed to PFOS had increased SMI compared to controls; salamanders did not demonstrate effects. Concentrations of 6:2 FTS in substrate decreased substantially by 30 d, likely driven by microbial action. Perfluorooctane sulfonate had notable biota–sediment accumulation factors, but was still <1. Although a no‐observable‐effect concentration could not generally be determined, the lowest‐observable‐effect concentration was 50 to 120 ppb. Survival was not affected. The present study demonstrates that PFAS bioaccumulation from dermal exposures and sublethal effects are dependent on species, chemical, and focal trait. Environ Toxicol Chem 2021;40:717–726. © 2020 SETAC

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皮肤接触多氟和全氟烷基物质对变形后两栖动物的亚致死效应

对多氟烷基和全氟烷基物质 (PFAS) 对两栖动物，特别是变态后的毒性的研究是有限的。我们研究了皮肤接触 PFAS（30 天）对美洲蟾蜍 ( Anaxyrus americanus )、东部虎蝾螈 ( Ambystoma tigrinum ) 和北豹蛙 ( Rana pipiens ) 的存活和生长的影响）。化学品包括全氟辛酸、全氟辛烷磺酸 (PFOS)、全氟己烷磺酸 (PFHxS) 和 6:2 氟调聚物磺酸盐 (6:2 FTS)，按苔藓干重计，浓度为 0、80、800 或 8000 ppb。接触 PFAS 会影响最终的口鼻部长度 (SVL) 和比例质量指数 (SMI)，后者是衡量相对身体状况的指标。观察到的影响取决于物种和化学物质，而不是浓度。与对照相比，暴露于 PFOS、PFHxS（仅限青蛙）和 6:2 FTS 的蝾螈显示 SVL 降低，而暴露于 6:2 FTS 的蝾螈则显示 SVL 增加。与对照组相比，暴露于全氟己烷磺酸和 6:2 FTS 的青蛙和暴露于全氟辛烷磺酸的蟾蜍的 SMI 增加；蝾螈没有表现出效果。底物中 6:2 FTS 的浓度在 30 天后显着降低，这可能是由微生物作用驱动的。全氟辛烷磺酸具有显着的生物群沉积物积累因子，但仍<1。虽然通常无法确定无观察到的影响浓度，但最低可观察到的影响浓度为 50 至 120 ppb。生存没有受到影响。本研究表明，来自皮肤接触和亚致死效应的 PFAS 生物积累取决于物种、化学和焦点特征。环境毒理学化学2021；40：717-726。© 2020 SETAC