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Identification and determination of selenocysteine, selenosugar, and other selenometabolites in turkey liver.
Metallomics ( IF 2.9 ) Pub Date : 2020-03-13 , DOI: 10.1039/d0mt00040j Bierla Katarzyna 1 , Rachel M Taylor , Joanna Szpunar , Ryszard Lobinski , Roger A Sunde
Metallomics ( IF 2.9 ) Pub Date : 2020-03-13 , DOI: 10.1039/d0mt00040j Bierla Katarzyna 1 , Rachel M Taylor , Joanna Szpunar , Ryszard Lobinski , Roger A Sunde
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Liver and other tissues accumulate selenium (Se) when animals are supplemented with high dietary Se as inorganic Se. To further study selenometabolites in Se-deficient, Se-adequate, and high-Se liver, turkey poults were fed 0, 0.4, and 5 μg Se g-1 diet as Na2SeO3 (Se(iv)) in a Se-deficient (0.005 μg Se g-1) diet for 28 days, and the effects of Se status determined using HPLC-ICP-MS and HPLC-ESI-MS/MS. No selenomethionine (SeMet) was detected in liver in turkeys fed either this true Se-deficient diet or supplemented with inorganic Se, showing that turkeys cannot synthesize SeMet de novo from inorganic Se. Selenocysteine (Sec) was also below the level of detection in Se-deficient liver, as expected in animals with negligible selenoprotein levels. Sec content in high Se liver only doubled as compared to Se-adequate liver, indicating that the 6-fold increase in liver Se was not due to increases in selenoproteins. What increased dramatically in high Se liver were low molecular weight (MW) selenometabolites: glutathione-, cysteine- and methyl-conjugates of the selenosugar, seleno-N-acetyl galactosamine (SeGalNac). Substantial Se in Se-adequate liver was present as selenosugars decorating general proteins via mixed-disulfide bonds. In high-Se liver, these "selenosugar-decorated" proteins comprised ∼50% of the Se in the water-soluble fraction, in addition to low MW selenometabolites. In summary, more Se is present as the selenosugar moiety in Se-adequate liver, mostly decorating general proteins, than is present as Sec in selenoproteins. With high Se supplementation, increased selenosugar formation occurs, further increasing selenosugar-decorated proteins, but also increasing selenosugar linked to low MW thiols.
中文翻译：
鉴定和测定火鸡肝脏中的硒代半胱氨酸，硒代糖和其他硒代代谢产物。
当向动物补充高膳食硒作为无机硒时，肝脏和其他组织中就会积聚硒。为了进一步研究硒缺乏，硒充足和高硒肝中的硒代谢产物，向火鸡家禽分别饲喂0、0.4和5μgSe g-1饮食作为Na2SeO3（Se（iv）），硒缺乏（0.005微克Se g-1）饮食28天，并使用HPLC-ICP-MS和HPLC-ESI-MS / MS确定硒状态的影响。在饲喂这种缺乏真正硒的饮食或补充无机硒的火鸡中，在肝脏中未检测到硒代蛋氨酸（SeMet），这表明火鸡无法从无机硒中重新合成SeMet。硒缺乏的肝脏中的硒代半胱氨酸（Sec）也低于检测水平，这与硒蛋白水平可忽略的动物所期望的一样。高硒肝脏中的硒含量仅比硒充足的肝脏高一倍，这表明肝脏硒的6倍增加不是由于硒蛋白的增加。高硒肝中显着增加的是低分子量（MW）硒代代谢物：硒代糖的谷胱甘肽，半胱氨酸和甲基结合物，硒代N-乙酰半乳糖胺（SeGalNac）。硒充足的肝脏中大量硒作为硒糖通过混合二硫键修饰普通蛋白质。在高硒肝中，这些“硒糖修饰的”蛋白质除低分子量硒代谢产物外，还占水溶性部分中硒的约50％。总之，在硒充足的肝脏中，作为硒代糖部分存在的硒要多于硒蛋白中作为硒化物的硒，主要是修饰普通蛋白质。补充高硒会增加硒糖的形成，进一步增加硒糖修饰的蛋白质，
更新日期：2020-03-13
中文翻译：
鉴定和测定火鸡肝脏中的硒代半胱氨酸，硒代糖和其他硒代代谢产物。
当向动物补充高膳食硒作为无机硒时，肝脏和其他组织中就会积聚硒。为了进一步研究硒缺乏，硒充足和高硒肝中的硒代谢产物，向火鸡家禽分别饲喂0、0.4和5μgSe g-1饮食作为Na2SeO3（Se（iv）），硒缺乏（0.005微克Se g-1）饮食28天，并使用HPLC-ICP-MS和HPLC-ESI-MS / MS确定硒状态的影响。在饲喂这种缺乏真正硒的饮食或补充无机硒的火鸡中，在肝脏中未检测到硒代蛋氨酸（SeMet），这表明火鸡无法从无机硒中重新合成SeMet。硒缺乏的肝脏中的硒代半胱氨酸（Sec）也低于检测水平，这与硒蛋白水平可忽略的动物所期望的一样。高硒肝脏中的硒含量仅比硒充足的肝脏高一倍，这表明肝脏硒的6倍增加不是由于硒蛋白的增加。高硒肝中显着增加的是低分子量（MW）硒代代谢物：硒代糖的谷胱甘肽，半胱氨酸和甲基结合物，硒代N-乙酰半乳糖胺（SeGalNac）。硒充足的肝脏中大量硒作为硒糖通过混合二硫键修饰普通蛋白质。在高硒肝中，这些“硒糖修饰的”蛋白质除低分子量硒代谢产物外，还占水溶性部分中硒的约50％。总之，在硒充足的肝脏中，作为硒代糖部分存在的硒要多于硒蛋白中作为硒化物的硒，主要是修饰普通蛋白质。补充高硒会增加硒糖的形成，进一步增加硒糖修饰的蛋白质，
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