LED array microscopy is an emerging platform for computational imaging with significant utility for biological imaging. Existing LED array systems often exploit transmission imaging geometries of standard brightfield microscopes that leave the rich backscattered field undetected. This backscattered signal contains high-resolution sample information with superb sensitivity to subtle structural features that make it ideal for biological sensing and detection. Here, we develop an LED array reflectance microscope capturing the sample's backscattered signal. In particular, we demonstrate multimodal brightfield, darkfield, and differential phase contrast imaging on fixed and living biological specimens including Caenorhabditis elegans (C. elegans), zebrafish embryos, and live cell cultures. Video-rate multimodal imaging at 20 Hz records real time features of freely moving C. elegans and the fast beating heart of zebrafish embryos. Our new reflectance mode is a valuable addition to the LED array microscopic toolbox.

中文翻译：

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LED阵列反射显微镜用于基于散射的多对比度成像。

LED阵列显微镜是一种新兴的计算成像平台，对生物成像具有重要的用途。现有的LED阵列系统通常利用标准明场显微镜的透射成像几何形状，从而无法检测到丰富的反向散射场。该反向散射信号包含高分辨率样本信息，并且对微妙的结构特征具有极高的灵敏度，使其非常适合生物传感和检测。在这里，我们开发了一种LED阵列反射显微镜，用于捕获样品的反向散射信号。特别是，我们展示了在固定的和活的生物样本（包括秀丽隐杆线虫（C. elegans），斑马鱼胚胎和活细胞培养物）上的多峰明场，暗场和差分相衬成像。20 Hz的视频速率多模态成像记录了自由移动的秀丽隐杆线虫和斑马鱼胚胎快速跳动的心脏的实时特征。我们的新反射模式是LED阵列微观工具箱的宝贵补充。