Abstract

Natural polymeric proteins, isolated from scallop gonad, had already shown promise as a potent nutraceutical. The purpose of this study was to fabricate scallop gonad protein isolates (SGPIs) as a natural alternative emulsifier designed for improving the physicochemical stability of β-carotene (BC). The influence of carrier oil, including long- and medium-chain triglycerides (LCT/MCT), on the physicochemical stability of BC emulsions was investigated. The SGPI-stabilized BC emulsions were characterized in terms of viscosity, particle diameter, zeta-potential, atomic force microscopy (AFM), cryogenic scanning electron microscopy (cryo-SEM), and confocal laser scanning microscopy (CLSM). Compared to SGPI-MCT emulsions, SGPI-LCT emulsions had better resistance to high ionic strengths and thermal treatment. During the 30-day storage period, the particle diameter, zeta-potential, and BC degradation of both emulsions had a great change at 37 °C than at 25 or 4 °C, and the variation of SGPI-MCT emulsions was more obvious than that of SGPI-LCT, which could be attributed to the high viscosity of SGPI-LCT emulsions. These results indicate that SGPIs can be applied in protecting lipophilic bioactives by using emulsion-based systems, in which LCT oil was more effective for encapsulating and protecting BC than MCT oil.

Graphical abstract

中文翻译：

---

扇贝（Patinopecten yessoensis）Gonad蛋白分离物在基于乳液的输送系统中保护β-胡萝卜素免受化学降解

摘要

从扇贝性腺中分离出的天然高分子蛋白已经显示出作为强效保健食品的前景。这项研究的目的是制造扇贝性腺蛋白分离物（SGPIs）作为设计用于改善β-胡萝卜素（BC）的理化稳定性的天然替代乳化剂。研究了包括长链和中链甘油三酸酯（LCT / MCT）在内的载油对BC乳液理化稳定性的影响。SGPI稳定的BC乳液通过粘度，粒径，ζ电位，原子力显微镜（AFM），低温扫描电子显微镜（cryo-SEM）和共聚焦激光扫描显微镜（CLSM）进行了表征。与SGPI-MCT乳液相比，SGPI-LCT乳液对高离子强度和热处理具有更好的抵抗力。在30天的存储期内，与25°C或4°C相比，两种乳液在37°C时的粒径，ζ电势和BC降解都有很大的变化，并且SGPI-MCT乳液的变化比SGPI-LCT更明显，这可以归因于SGPI-LCT乳液的高粘度。这些结果表明，SGPIs可以通过使用基于乳液的系统应用于保护亲脂性生物活性物质，其中LCT油比MCT油更有效地包裹和保护BC。