Abstract

Microalgae crops targeting biotechnological applications are conducted in photobioreactors, which allow the adjustment and control of parameters of luminosity, agitation and mixing in the cultivation medium to promote better cell growth and accumulation of metabolites. In this sense, the present work used the Taguchi method to find the best adjustment of the operational parameters of an unconventional photobioreactor denominated internally illuminated integrated photobioreactor (ILI-PBR) with LED light aiming at elevating the biomass concentration, volumetric biomass productivity, and volumetric lipid productivity of Chlorella minutissima microalgae cultivated under autotrophic regime. The effects of the factors were evaluated: illumination (blue, white and red); photoperiod (12 h light:12 h dark, 1 h light:1 h dark, and 24 h clear:0 h dark); aeration (0, 3 vvm, 0.4 vvm, and 0.5 vvm); and recirculation flow rate of cultivation medium (5 L min⁻¹, 6.5 L min⁻¹, and 9 L min⁻¹) on the variable responses: biomass concentration, biomass volumetric productivity, lipid content, and volumetric lipid productivity. The use of the Taguchi method allowed the increase of biomass concentration, volumetric biomass productivity, and volumetric lipid productivity in the biomass of Chlorella minutissimain 8.6%, 42%, and 143%, respectively, with the adjustment of the operational parameters of the photobioreactor used.

中文翻译：

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使用Taguchi方法，对小球藻的分批自养培养进行非常规集成和照明内部光生物反应器（ILI-PBR）的操作参数调整。

摘要

针对生物技术应用的微藻作物在光生物反应器中进行，从而可以调节和控制培养基中的光度，搅拌和混合参数，以促进更好的细胞生长和代谢产物积累。从这个意义上讲，目前的工作使用田口方法来寻找以LED照明的非常规光生物反应器内部照明集成光生物反应器（ILI-PBR）的运行参数的最佳调整，旨在提高生物量浓度，体积生物量生产率和体积小球藻的脂质生产力在自养条件下培养的微藻。评估了这些因素的影响：照明（蓝色，白色和红色）；光周期（12小时光照：12小时黑暗，1小时光照：1小时黑暗和24小时清除：0小时黑暗）; 曝气（0、3 vvm，0.4 vvm和0.5 vvm）; 和培养培养基（5L分钟的再循环流速^-1，6.5升分钟^-1，和9升分钟^-1）上的可变响应：生物质浓度，生物质体积生产率，脂质含量，和体积脂质生产力。Taguchi方法的使用可以提高小球藻的生物量中的生物量浓度，体积生物量生产力和脂质体生产力调整所用光生物反应器的操作参数后，分别达到8.6％，42％和143％。