CD45, a common leukocyte antigen expressed on the surface of all nucleated hematopoietic cells, indicates the developmental stage and functional status of lymphocytes by its alternative splicing isoforms. Estrogen is correlated with the immune activity of lymphocytes and is involved in the sex bias of several human autoimmune diseases, but the effect of estrogen on the expression of the CD45 splicing isoforms remains unknown. In the present study, a potential estrogen response element was identified on the opposite strand of the CD45 gene by bioinformatics software prediction. The results from RT-qPCR results showed that the expression levels of CD45RO isoform and CD45 antisense RNA were increased after the lymphocytes were treated with 10 nM 17beta-estradiol, and this effect of 17beta-estradiol was reversed when the lymphocytes were cotreated with an estrogen receptor antagonist. Moreover, bisulfite sequencing PCR showed that CD45 DNA methylation in lymphocytes was increased after the treatment with 10 nM 17beta-estradiol. In conclusion, estradiol regulated the expression of CD45 in an estrogen receptor-dependent manner and was associated with CD45 antisense RNA and DNA methylation. The results helped elucidate the regulatory mechanism of the expression of CD45 isoforms and the correlation between estrogen levels and immune activity in females.

中文翻译：

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雌二醇调节淋巴细胞中CD45剪接异构体的表达。

CD45是一种表达在所有有核造血细胞表面的常见白细胞抗原，它通过其可变的剪接同工型指示淋巴细胞的发育阶段和功能状态。雌激素与淋巴细胞的免疫活性相关，并参与几种人类自身免疫性疾病的性别偏见，但雌激素对CD45剪接同工型表达的影响尚不清楚。在本研究中，通过生物信息学软件预测，在CD45基因的相反链上鉴定出了潜在的雌激素反应元件。RT-qPCR结果表明，用10nM17β-雌二醇处理淋巴细胞后，CD45RO亚型和CD45反义RNA的表达水平增加，当用雌激素受体拮抗剂共同处理淋巴细胞时，17β-雌二醇的这种作用被逆转。此外，亚硫酸氢盐测序PCR显示，用10 nM17β-雌二醇处理后，淋巴细胞中CD45 DNA甲基化增加。总之，雌二醇以雌激素受体依赖性方式调节CD45的表达，并与CD45反义RNA和DNA甲基化有关。这些结果有助于阐明女性CD45亚型表达的调控机制以及雌激素水平与免疫活性之间的关系。雌二醇以雌激素受体依赖性方式调节CD45的表达，并与CD45反义RNA和DNA甲基化有关。这些结果有助于阐明女性CD45亚型表达的调控机制以及雌激素水平与免疫活性之间的关系。雌二醇以雌激素受体依赖性方式调节CD45的表达，并与CD45反义RNA和DNA甲基化有关。这些结果有助于阐明女性CD45亚型表达的调控机制以及雌激素水平与免疫活性之间的关系。