Purpose

To assess estradiol (E₂) and progesterone levels during ovarian stimulation determined by third-generation (Gen III) and second-generation (Gen II) Elecsys^® immunoassays.

Methods

E₂ and progesterone concentrations were measured using Elecsys^® Gen III and Gen II immunoassays, and progesterone concentrations on the day of ovulation triggering were determined by LC–MS/MS. This was a retrospective, non-interventional study conducted at European tertiary referral infertility clinics in women aged 18–45 years, with a body mass index 18–35 kg/m², regular menses, and both ovaries.

Results

Serum samples were obtained from 230 women classified by oocyte retrieval as poor (33.0%; 0–3 oocytes), normal (40.9%; 4–15 oocytes), or high (26.1%; > 15 oocytes) responders. E₂ and progesterone levels increased during ovarian stimulation, with greatest increases observed in high responders. Elecsys^® Gen III and Gen II assay results were highly correlated for E₂ (Pearson’s r = 0.99) and progesterone (r = 0.89); Gen III results were lower than Gen II for both E₂ and progesterone. On the day of triggering, Gen III E₂ and progesterone levels showed a difference of − 15.0% and − 27.9%, respectively. Progesterone levels (on day of triggering) measured by LC–MS/MS correlated better with Gen III (0.98) than Gen II (0.90). Mean relative differences for Gen III and Gen II assays versus LC–MS/MS were 14.6% and 62.8%, respectively.

Conclusion

E₂ and progesterone levels determined with Elecsys^® Gen II and III assays were highly correlated; results were lower for Gen III versus Gen II. Differences observed for progesterone on the day of triggering may be clinically relevant.

中文翻译：

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体外受精中的雌二醇和孕甾酮：一项评估第三代与第二代雌二醇和孕酮免疫测定的多中心研究。

目的

为了评估雌二醇（E ₂）和卵巢刺激期间孕酮水平来确定由第三代（第三代）和第二代（第二代）Elecsys测定^®免疫测定。

方法

ë ₂和孕酮浓度使用Elecsys测定测定^®第三代和第二代免疫测定法，和对排卵日孕酮浓度触发通过LC-MS / MS测定。这是在欧洲三级转诊不育诊所针对18-45岁，体重指数18-35 kg / m ²，月经正常和双卵巢的女性进行的一项回顾性非干预性研究。

结果

血清样本来自230名按卵母细胞回收分类为不良（33.0％； 0–3个卵母细胞），正常（40.9％； 4–15个卵母细胞）或高（26.1％；> 15个卵母细胞）应答者。E ₂和孕激素水平在卵巢刺激过程中增加，在高反应者中观察到最大的增加。Elecsys测定^®第三代和第二代测定法的结果高度对于E相关₂（皮尔森- [R  = 0.99）和孕激素（[R  = 0.89）; 对于E ₂和孕激素，第三代结果均低于第二代。在触发之日，第三代E ₂和孕酮水平分别显示出-15.0％和-27.9％的差异。LC-MS / MS测定的孕激素水平（在触发当天）与Gen III（0.98）的相关性高于Gen II（0.90）。Gen III和Gen II分析相对于LC-MS / MS的平均相对差异分别为14.6％和62.8％。

结论

ë ₂和孕酮水平来确定与Elecsys测定^®代II和III的测定是高度相关; 第三代的结果比第二代的要低。在触发当天观察到的孕酮差异可能与临床有关。