INTRODUCTION Sepsis is a leading cause of mortality in burn patients. One of the major causes of sepsis in burn patients is Pseudomonas aeruginosa. We hypothesized that during dissemination from infected burn wounds and subsequent sepsis, P. aeruginosa affects the metabolome of the blood resulting in changes to specific metabolites that would serve as biomarkers for early diagnosis of sepsis caused by P. aeruginosa. OBJECTIVES To identify specific biomarkers in the blood after sepsis caused by P. aeruginosa infection of burns. METHODS Gas chromatography with time-of-flight mass spectrometry was used to compare the serum metabolome of mice that were thermally injured and infected with P. aeruginosa (B-I) to that of mice that were neither injured nor infected, mice that were injured but not infected, and mice that were infected but not injured. RESULTS Serum levels of 19 metabolites were significantly increased in the B-I group compared to controls while levels of eight metabolites were significantly decreased. Thymidine, thymine, uridine, and uracil (related to pyrimidine metabolism), malate and succinate (a possible sign of imbalance in the tricarboxylic acid cycle), 5-oxoproline (related to glutamine and glutathione metabolism), and trans-4-hydroxyproline (a major component of the protein collagen) were increased. Products of amino acid metabolism were significantly decreased in the B-I group, including methionine, tyrosine, indole-3-acetate, and indole-3-propionate. CONCLUSION In all, 26 metabolites were identified, including a unique combination of five metabolites (trans-4-hydroxyproline, 5-oxoproline, glycerol-3-galactoside, indole-3-acetate, and indole-3-propionate) that could serve as a set of biomarkers for early diagnosis of sepsis caused by P. aeruginosa in burn patients.

中文翻译：

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烧伤感染期间由铜绿假单胞菌引起的败血症的新标志物。

简介脓毒症是烧伤患者死亡的主要原因。烧伤患者败血症的主要原因之一是铜绿假单胞菌。我们假设在从感染的烧伤伤口和随后的脓毒症传播期间，铜绿假单胞菌会影响血液的代谢组，导致特定代谢产物发生变化，从而可以作为早期诊断由铜绿假单胞菌引起的败血症的生物标记。目的鉴定铜绿假单胞菌烧伤感染引起的败血症后血液中的特定生物标志物。方法采用飞行时间质谱气相色谱法比较热损伤和感染铜绿假单胞菌（BI）的小鼠的血清代谢组与既未受伤也未感染的小鼠，未受伤但未感染的小鼠的血清代谢组被感染，以及被感染但未受伤的小鼠。结果与对照组相比，BI组的血清19种代谢物水平显着增加，而8种代谢物的水平显着下降。胸腺嘧啶，胸腺嘧啶，尿苷和尿嘧啶（与嘧啶代谢有关），苹果酸和琥珀酸酯（可能在三羧酸循环中失衡的信号），5-氧代脯氨酸（与谷氨酰胺和谷胱甘肽代谢有关）和反式-4-羟基脯氨酸蛋白质胶原蛋白的主要成分）增加。BI组的氨基酸代谢产物显着降低，包括蛋氨酸，酪氨酸，3-吲哚乙酸酯和3-丙酸吲哚。结论总共鉴定出26种代谢物，包括五种代谢物的独特组合（反式4-羟基脯氨酸，5-氧代脯氨酸，甘油-3-半乳糖苷，吲哚-3-乙酸酯，