Abstract Fluorescamine (FSA) has been widely applied for the determination of several classes of compounds containing amino groups. The estimated substances included pharmaceutical compounds, pesticides, amino acids, peptides and proteins. In addition, the activities of some enzymes were also monitored by FSA assay. The employed techniques mainly included spectrofluorimetry, as spray reagent in TLC, a derivativzation reagent in HPLC and capillary electrophoresis. Although there are many other reagents for detecting primary amino group containing compounds, FSA is advantageous as the reagent itself and its hydrolytic product are non-fluorescent. While other reagents such as NBD-Cl, dansyl chloride and o-phthaldehyde have a native fluorescence and their hydrolytic products are indeed fluorescent. In addition, the involved reaction is very fast and carried out at ambient temperature. However, the sensitivity of FSA analysis is much lower than these reagents. The sensitivity of the reagent could be enhanced by the proper selection of the diluting solvent, addition of surfactants or extraction with water immiscible solvent. The present article represents a comprehensive review for the application of FSA as a reagent in chemical and biochemical analysis since its discovery until now.

中文翻译：

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使用荧光胺作为通用且方便的分析探针的综述

摘要 荧光胺 (FSA) 已被广泛应用于测定几类含氨基的化合物。估计的物质包括药物化合物、杀虫剂、氨基酸、肽和蛋白质。此外，一些酶的活性也通过 FSA 测定进行监测。所采用的技术主要包括荧光分光光度法，在薄层色谱中作为喷雾试剂，在高效液相色谱和毛细管电泳中作为衍生试剂。尽管有许多其他试剂可用于检测含伯氨基的化合物，但 FSA 的优势在于该试剂本身及其水解产物不发荧光。而其他试剂如 NBD-Cl、丹磺酰氯和邻苯二甲醛具有天然荧光，它们的水解产物确实具有荧光。此外，所涉及的反应非常快，并且在环境温度下进行。但是，FSA 分析的灵敏度远低于这些试剂。通过正确选择稀释溶剂，加入表面活性剂或用水不混溶溶剂，可以增强试剂的敏感性。本文对 FSA 作为化学和生化分析试剂自发现至今的应用进行了全面回顾。