In this study, the extraction, identification, and antialgal activity of toxic organic substances from decaying green alga Ulva prolifera, a disaster-causing alga on green tides in the Yellow Sea, were investigated. The toxic organic substances of U. prolifera were isolated by a sequence of procedures, namely, liquid-liquid extraction (LLE), silica gel chromatography column separation (SGCC), C18 solid phase extraction (C18 SPE), and acid-alkali separation (AAS) and their antialgal activities were tested against diatom Skeletonema costatum. The results showed that the 96h-EC₅₀ value in each procedure was 57.00 mg•L⁻¹, 12.14 mg•L⁻¹, 11.70 mg•L⁻¹, and 6.31 mg•L⁻¹ in turn. Additionally, the initial (ethyl acetate extract in LLE) and final (Organic phase A in AAS) yield of toxic organics were 318 mg•kg⁻¹ and 9 mg•kg⁻¹ (the wet weight of U. prolifera). The final active fractions were preliminary identified as five 16- and 18-carbon fatty acids and four enols using GC–MS. The study may further evaluate the effect of bioactive substances on marine life after U. prolifera green tides bloom.

在这项研究中，研究了从腐烂的绿色藻类Ulva prolifera（黄海中绿色潮汐中的致灾藻类）中有毒有机物质的提取，鉴定和抗藻活性。通过一系列程序分离U. prolifera的有毒有机物，即液-液萃取（LLE），硅胶色谱柱分离（SGCC），C18固相萃取（C18 SPE）和酸-碱分离（ AAS）及其抗藻活性针对硅藻骨骼肌进行了测试。结果表明，96小时的-EC ₅₀在每个过程值为57.00毫克•L ^-1，12.14毫克•L ^-1，11.70毫克•L ^-1，依次为6.31 mg•L ^-1。另外，有毒有机物的初始（LLE中的乙酸乙酯提取物）和最终（AAS中的有机相A）的产率分别为318 mg•kg ^-1和9 mg•kg ^-1（U。prolifera的湿重）。最终的活性成分通过GC-MS初步鉴定为5个16-和18-碳脂肪酸和4个烯醇。这项研究可能会进一步评估生物活性物质对美国增殖绿潮开花后海洋生物的影响。