Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities.

中文翻译：

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使用CRISPR / Cas9从人类胚胎干细胞生成TLR7杂合敲除系（WAe009-A-18）。

Toll样受体7（TLR7）是Toll样受体（TLR）家族的成员，在先天免疫系统中至关重要。在这项研究中，我们使用CRISPR / Cas9建立了杂合的TLR7基因敲除H9细胞系。TLR7基因敲除H9细胞保持其多能性，并表现出分化为三个胚层的能力，而没有任何核型异常。