Abstract Meat authenticity testing along food supply chain can be largely improved through fast and integrated DNA-based identification methods. A species-specific polymerase chain reaction (PCR) coupled with lateral flow immunoassay (LFI) was developed for the identification of goose meat. The goose-specific primers that could specifically enrich a fragment on mitochondrial 12s RNA gene were successfully designed. The specificity of the primers was verified against 19 other species associated with meat fraud. The proposed PCR-LFI device was able to detect 0.01% (w/w) of goose in uncooked binary mixtures and 0.1% (w/w) in cooked binary mixtures. The LFI strip as a post-PCR visualization method was more sensitive and faster than gel electrophoresis method. Eight collaborative laboratories verified the reproducibility of fabrication and detection protocols of the developed LFI. This PCR-LFI method has great potential to be used as a fast and cost-efficient counter-measure for food industry and other regulatory bodies to reduce the possibility of fraud of goose products.

中文翻译：

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物种特异性 PCR 结合侧流免疫分析法用于鉴定食品中鹅成分的开发

摘要 通过基于 DNA 的快速集成识别方法，可以在很大程度上改进食品供应链中的肉类真实性检测。开发了一种物种特异性聚合酶链反应 (PCR) 与侧流免疫测定 (LFI) 相结合的方法，用于鉴定鹅肉。成功设计了能够特异性富集线粒体12s RNA基因片段的鹅特异性引物。针对与肉类欺诈相关的 19 种其他物种验证了引物的特异性。提议的 PCR-LFI 设备能够检测到未煮熟的二元混合物中 0.01% (w/w) 的鹅和煮熟的二元混合物中的 0.1% (w/w)。作为 PCR 后可视化方法的 LFI 条比凝胶电泳方法更灵敏和更快。八个合作实验室验证了开发的 LFI 的制造和检测协议的可重复性。这种 PCR-LFI 方法具有巨大的潜力，可用作食品工业和其他监管机构的快速且具有成本效益的对策，以减少鹅产品欺诈的可能性。