Abstract The objective of this study was to assess the influence of pressure-assisted thermal sterilization (PATS) on nutrients and quality of pumpkin, butternut squash, pea, beetroot, and purple potato purees. PATS processing parameters included preheating of purees at 98 °C for 5 min and pressurizing at 600 MPa in a vessel set at 90 °C for 5 min. Colorimetric temperature sensors, two types of Bacillus amyloliquefaciens spores, total aerobic and anaerobic plate count, HPLC, as well as spectrometer and spectrophotometer were utilized. The colorimetric temperature sensor based on its color value when extrapolated suggested the purees' temperature reached 122 °C. Thus, at least 9-log10 reduction of B. amyloliquefaciens spores was achieved in a purple potato puree, and no detectable aerobic and anaerobic microorganisms were found after PATS in all purees. Vitamin C showed 3–14% reduction after PATS. Total chlorophyll and β-carotene did not vary significantly (p > 0.05) after PATS, while betalains and anthocyanin were sensitive and decreased significantly. Interestingly, total color change (ΔE) of pumpkin, butternut squash, and beetroot purees ranged between 2.5 and 3.4 below the threshold (ΔE = 6) value that can be observed by the naked eyes. EVOH-based packages did not show any visual deformation, but their oxygen and water vapor transmission rates increased significantly (p

中文翻译：

---

低酸果蔬泥的高压辅助热杀菌：微生物安全、营养、质量和包装评价

摘要 本研究的目的是评估压力辅助热灭菌 (PATS) 对南瓜、胡桃南瓜、豌豆、甜菜根和紫薯泥的营养成分和质量的影响。PATS 加工参数包括在 98°C 下预热果泥 5 分钟，并在设置为 90°C 的容器中以 600 MPa 加压 5 分钟。使用比色温度传感器、两种解淀粉芽孢杆菌孢子、总需氧和厌氧平板计数、HPLC，以及光谱仪和分光光度计。比色温度传感器基于其外推时的颜色值表明果泥的温度达到 122 °C。因此，在紫色马铃薯泥中，解淀粉芽孢杆菌孢子至少减少了 9-log10，PATS 处理后，所有果泥中均未发现可检测的需氧和厌氧微生物。PATS 后维生素 C 减少了 3-14%。PATS后总叶绿素和β-胡萝卜素没有显着变化（p> 0.05），而甜菜碱和花青素敏感且显着降低。有趣的是，南瓜、胡桃南瓜和甜菜根泥的总颜色变化 (ΔE) 比肉眼可以观察到的阈值 (ΔE = 6) 低 2.5 到 3.4 之间。基于 EVOH 的包装没有显示任何视觉变形，但它们的氧气和水蒸气透过率显着增加（p 南瓜、胡桃南瓜和甜菜根泥的总颜色变化 (ΔE) 范围在 2.5 到 3.4 之间，低于肉眼可以观察到的阈值 (ΔE = 6)。基于 EVOH 的包装没有显示任何视觉变形，但它们的氧气和水蒸气透过率显着增加（p 南瓜、胡桃南瓜和甜菜根泥的总颜色变化 (ΔE) 范围在 2.5 到 3.4 之间，低于肉眼可以观察到的阈值 (ΔE = 6)。基于 EVOH 的包装没有显示任何视觉变形，但它们的氧气和水蒸气透过率显着增加（p