To elucidate the role of long alkyl group in sulfated poly- and oligosaccharides on anti-HIV activity, the interaction between sulfated 3-O-octadecyl-(1→6)-α-d-glucopyranan with potent anti-HIV activity and liposomes with diameters of 58 ± 20 nm and 107 ± 28 nm as models of HIV were investigated. SPR measurements of sulfated 3-O-octadecyl-(1→6)-α-d-glucopyranans bearing 2.8 mol% of the octadecyl group and the liposome (diameter = 58 ± 20.0 nm and ζ=0 mV) resulted in an apparent association- ka = 6 × 105 1/M, a dissociation-rate kd = 4 × 10-4 1/s, and a dissociation constants KD = 8 × 10-10 M. The particle size of the sulfated 3-O-octadecyl-(1→6)-α-d-glucopyranan (67 ± 14 nm) measured by DLS increased to 104 ± 25 nm, whereas the ζ potential (-29 mV) was unchanged (-33 mV). For dextran sulfate without an alkyl group, no interaction was observed. These results suggest that the long octadecyl group penetrated into the liposome and sulfated glucopyranan was covered on the liposome to increase the anti-HIV activity. The 107 nm liposome exhibited similar results.

中文翻译：

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表面等离振子共振分析了硫酸化3-O-十八烷基-α-（1→6）-d-葡聚糖与脂质体之间的相互作用。

为了阐明硫酸化多糖和寡糖中的长烷基对抗HIV活性的作用，具有较强抗HIV活性的硫酸化3-O-十八烷基-（1→6）-α-d-吡喃葡聚糖与脂质体之间的相互作用研究了直径为58±20 nm和107±28 nm的HIV模型。带有2.8摩尔％十八烷基和脂质体（直径= 58±20.0 nm和ζ= 0 mV）的硫酸化3-O-十八烷基-（1→6）-α-d-吡喃吡喃的SPR测量导致明显的缔合-ka = 6×105 1 / M，解离速率kd = 4×10-4 1 / s，解离常数KD = 8×10-10M。硫酸化3-O-十八烷基-用DLS测定的（1→6）-α-d-吡喃吡喃（67±14 nm）增加到104±25 nm，而ζ电位（-29 mV）不变（-33 mV）。对于没有烷基的硫酸葡聚糖，没有观察到相互作用。这些结果表明长的十八烷基基团渗透到脂质体中，并且硫酸化的吡喃葡聚糖被脂质体覆盖以增加抗HIV活性。107 nm脂质体显示出相似的结果。