Free lead and zinc ions are toxic to living cells and environment, we develop a multi-responsive fluorescence probe based on water-soluble double hydrophilic block copolymer. The polymer sensor with Schiff moiety can sensitively, selectively and simultaneously fluorescence probe Zn²⁺ (green) and Pb²⁺ (blue) through the different fluorescence color. The linear concentration ranges for Pd²⁺ and Zn²⁺ were 0.8–10.0 μM and 0–10.0 μM with the detection limits of 0.7 μM and 0.2 μM, respectively. The probe responding to Na⁺, Mn²⁺, Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺, Cd²⁺, Al³⁺, Cr³⁺, Co²⁺, Ni²⁺, Ag⁺, Ca²⁺, K⁺, Mg²⁺, Ba²⁺, Hg²⁺, and Pb²⁺ displays almost no significant interference with the assay process. The sensing mechanism of the PSAM probe to Pb²⁺ and Zn²⁺ was investigated by Job's plot experiment, ¹H NMR analysis and density functional theory (DFT), the results show that the nitrogen atom of CHN and oxygen atom of OH may recognize with ions and the Job's plots for the PSAM complexes demonstrate 1:1 complexation, respectively. To achieve its application, the chemosensor is successfully applied to monitor Pd²⁺and Zn²⁺ in tap and lake water samples, its ability to monitor free Pd²⁺ and Zn²⁺ ions in living cells or in vivo is also validated by the intracellular imaging.

游离铅和锌离子对活细胞和环境均具有毒性，我们基于水溶性双亲水嵌段共聚物开发了一种多响应荧光探针。具有席夫部分的聚合物传感器可以通过不同的荧光颜色灵敏，选择性地同时荧光探针Zn ²⁺（绿色）和Pb ²⁺（蓝色）。Pd ²⁺和Zn ²⁺的线性浓度范围为0.8-10.0μM和0-10.0μM，检出限分别为0.7μM和0.2μM。探针响应Na ⁺，Mn 2 ⁺，Fe 2 ⁺，Fe ³⁺，Cu 2 ⁺，Zn 2 ⁺，Cd 2 ⁺，Al³⁺，Cr ³⁺，Co 2 ⁺，Ni 2 ⁺，Ag ⁺，Ca 2 ⁺，K ⁺，Mg 2 ⁺，Ba 2 ⁺，Hg ²⁺和Pb ²⁺几乎不影响分析过程。通过Job's作图实验，¹ H NMR分析和密度泛函理论（DFT）研究了PSAM探针对Pb ²⁺和Zn ²⁺的传感机理，结果表明CH的氮原子OH的N和氧原子可以被离子识别，PSAM络合物的Job's图分别显示1：1络合。为了实现其应用中，化学传感器被成功地应用于监视器PD ²⁺和Zn ²⁺抽头和湖泊水样中，其监测不含钯能力²⁺和Zn ²⁺离子在活细胞中或在体内也由验证细胞内成像。