A hydrodynamic snare and well array were developed to accomplish single-cell trapping and stem-cell perfusion culture. Directed streamline distribution is induced using unique V-shaped weir to load single bone marrow stem cell into U-shaped dwelling weir. The voids decrease flow speed in the dwelling area and enable a stable perfusion culture. The hydrodynamic snaring array was integrated with an on-line temperature control and monitoring system, and the ultraviolet illumination effect on a microscope is eliminated that is suitable for the long-term growth of single bone marrow stem cell with minimal external interventions.

中文翻译：

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用于单细胞诱捕和灌注培养的流体动力感应阵列

开发了流体动力圈套器和孔阵列以完成单细胞捕获和干细胞灌注培养。使用独特的V形堰将定向的流线分布诱导为将单个骨髓干细胞加载到U形居住堰中。空隙降低了居住区域中的流速并实现了稳定的灌注培养。流体动力感应阵列与在线温度控制和监控系统集成在一起，消除了在显微镜上的紫外线照射效果，该效果适合于单个骨髓干细胞的长期生长，而外部干预最少。