Mollisia is a taxonomically neglected discomycete genus (Helotiales, Leotiomycetes) of commonly encountered saprotrophs on decaying plant tissues throughout temperate regions. The combination of indistinct morphological characters, more than 700 names in the literature, and lack of reference DNA sequences presents a major challenge when working with Mollisia. Unidentified endophytes, including strains that produced antifungal or antiinsectan secondary metabolites, were isolated from conifer needles in New Brunswick and placed with uncertainty in Phialocephala and Mollisia, necessitating a more comprehensive treatment of these genera. In this study, morphology and multigene phylogenetic analyses were used to explore the taxonomy of Mollisiaceae, including Mollisia, Phialocephala, and related genera, using new field collections, herbarium specimens, and accessioned cultures and sequences. The phylogeny of Mollisiaceae was reconstructed and compared using the nuc internal transcribed spacer rDNA (ITS) barcode and partial sequences of the 28S nuc rDNA (LSU) gene, largest subunit of RNA polymerase II (RPB1), DNA topoisomerase I (TOP1), and the hypothetical protein Lipin/Ned1/Smp2 (LNS2). The results show that endophytism is common throughout the Mollisiaceae lineage in a diverse range of hosts but is infrequently attributed to Mollisia because of a paucity of reference sequences. Generic boundaries within Mollisiaceae are poorly resolved and based on phylogenetic evidence the family included species placed in Acephala, Acidomelania, Barrenia, Bispora, Cheirospora, Cystodendron, Fuscosclera, Hysteronaevia, Loramyces, Mollisia, Neopyrenopeziza, Obtectodiscus, Ombrophila, Patellariopsis, Phialocephala, Pulvinata, Tapesia (=Mollisia), and Trimmatostroma. Taxonomic novelties included the description of five novel Mollisia species and five novel Phialocephala species and the synonymy of Fuscosclera with Phialocephala, Acidomelania with Mollisia, and Loramycetaceae with Mollisiaceae.

Mollisia是分类学上被忽视的盘菌属（ Helotiales、Leotiomycetes ），是整个温带地区腐烂植物组织中常见的腐生菌。模糊的形态特征、文献中超过 700 个名称以及缺乏参考 DNA 序列的结合给Mollisia的研究带来了重大挑战。从新不伦瑞克省的针叶树针中分离出不明的内生菌，包括产生抗真菌或抗昆虫次级代谢产物的菌株，并不确定地放置在Phialocephala和Mollisia中，需要对这些属进行更全面的处理。在这项研究中，利用新的野外收藏品、植物标本以及加入的培养物和序列，利用形态学和多基因系统发育分析来探索莫里西亚科（Mollisiaceae）的分类学，包括莫里西亚（Mollisia ）、花头藻（Phialocephala）和相关属。使用 nuc 内部转录间隔区 rDNA (ITS) 条形码和 28S nuc rDNA ( LSU) 基因的部分序列、RNA 聚合酶 II (RPB1 )的最大亚基、DNA 拓扑异构酶 I ( TOP1 ) 和假设的蛋白质 Lipin/Ned1/Smp2 ( LNS2 )。结果表明，内生现象在不同宿主的整个软体科谱系中很常见，但由于参考序列的缺乏，很少归因于软体属。 Mollisiaceae内的属属边界尚未确定，根据系统发育证据，该科包括Acephala 、 Acidomelania 、 Barrenia 、 Bispora 、 Cheirospora 、 Cystodendron 、 Fuscosclera 、 Hysteronaevia 、 Loramyces 、 Mollisia 、 Neopyrenopeziza 、 Obtectodiscus 、 Ombrophila 、 Patellariopsis 、 Phialocephala 、维纳塔, Tapesia （= Mollisia ）和Trimmatostroma。分类学上的新颖之处包括对五种新的Mollisia物种和五种新的Phialocephala物种的描述，以及Fuscosclera与Phialocephala的同义， Acidomelania与Mollisia 的异名，以及Loramycetaceae与Mollisiaceae 的异名。