Circadian rhythms are endogenous oscillations of approximately 24-h periods and regulate diel changes in physiological biochemical processes. We examined the effects of black ginger (Kaempferia parviflora) on the expression of circadian clock genes and the disruption of circadian rhythms using in vitro and in vivo assays. K. parviflora extract increased the amplitude of circadian rhythms, extended the circadian period, and caused phase delays in NIH3T3 cells. We identified chemical components involved in the circadian clock gene expression using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance. 3,5,7,3′,4′-Pentamethoxyflavone, 5,7,4′-trimethoxyflavone, and 5,7-dimethoxyflavone were identified as components that regulated the circadian clock gene expression. In addition, we examined the effects of K. parviflora on behavioral entrainment in mice subjected to experimental jet lag (6-h phase advance), finding that K. parviflora enhanced adaptation to jet lag. These results suggested that polymethoxyflavones in K. parviflora could regulate the circadian clock and reduce circadian disruption.

昼夜节律是大约24小时周期的内源性振荡，可调节生理生化过程中的di​​el变化。我们使用体外和体内试验研究了黑姜（Kaempferia parviflora）对昼夜节律基因表达和昼夜节律破坏的影响。小花K. parviflora提取物增加了昼夜节律的幅度，延长了昼夜节律的周期，并导致NIH3T3细胞的相位延迟。我们使用高分辨率电喷雾电离质谱和核磁共振技术确定了生物钟基因表达中涉及的化学成分。3,5,7,3'，4'-五甲氧基黄酮，5,7,4'-三甲氧基黄酮和5,7-二甲氧基黄酮被确定为调节昼夜节律基因表达的成分。此外，我们检查了K. parviflora对实验性时差反应（6-h相提前）小鼠的行为夹带的影响，发现K. parviflora增强了对时差反应的适应性。这些结果表明，K。parviflora中存在聚甲氧基黄酮。 可以调节生物钟并减少生物钟的破坏。