This study was performed to reveal mitochondria changes and metabolome differences between bovine longissimus lumborum (LL) and psoas major (PM) muscles within the first 24 h postmortem. PM showed significant lower (P < .05) pH than LL at 6 h and 12 h postmortem. LL had significant higher (P < .05) mitochondrial membrane potential and lower mitochondrial reactive oxygen species levels than PM at 24 h postmortem. For metabolome, distinctive separation between LL and PM could be seen from principal component analysis and hierarchical clustering analysis. Carnosine, L-histidine and adenosine diphosphate were found with higher abundances in LL. Whereas higher abundances of inosine and hypoxanthine were detected in PM, which indicated distinct purine metabolism rate between muscle types. Higher abundances of glycerophospholipids in PM may increase risk of oxidation damage, and subsequently result in inferior meat quality during extended retail display. The results of this study provided an idea for further research regarding the muscle-specific mitochondrial ROS on meat quality.

进行这项研究是为了揭示死后头24小时内牛腰最长肌（LL）和腰大肌（PM）肌肉之间的线粒体变化和代谢组差异。PM显着降低（P 死后6 h和12 h的pH值小于LL。死后24小时，LL具有比PM显着更高（P <.05）的线粒体膜电位和更低的线粒体活性氧水平。对于代谢组，从主成分分析和层次聚类分析可以看出LL和PM之间有明显的分离。发现肌肽，L-组氨酸和二磷酸腺苷在LL中具有较高的丰度。相比之下，PM中肌苷和次黄嘌呤的丰度更高，这表明不同类型肌肉之间的嘌呤代谢率明显不同。PM中甘油磷脂的含量较高可能会增加氧化损坏的风险，并因此在延长零售展示期间导致肉品质差。