Lignans are the bioactive constituents in Schisandra chinensis fruits. For the first time major representatives could directly be determined in plant extracts by using Supercritical Fluid Chromatography. Based on nine commercially available standards the method was developed, finally permitting their baseline separation in less than 10 min. The optimum setup showed to be a Viridis HSS C18 SB column, supercritical carbon dioxide and methanol. The compounds could be assigned in the extracts either at 210 nm or by MS, for which no modifications except of an additional sheath liquid (0.1 % acetic acid in methanol) were required. The determined lignan patterns were typical for S. chinensis, with schisandrol A being the most abundant compound, followed by schisandrin B or schisandrol B. As method validation results also complied well with the requirements the here presented method is definitely an interesting alternative to established techniques like UHPLC for the analysis of lignans in Schisandra chinensis.

中文翻译：

---

采用二极管阵列和质谱检测的超临界流体色谱法快速分析五味子中的九种木脂素。

木质素是五味子果实中的生物活性成分。首次可以通过使用超临界流体色谱法直接确定植物提取物中的主要代表。基于九种市售标准品，开发了该方法，最终使它们的基线分离时间不到10分钟。最佳设置显示为Viridis HSS C18 SB色谱柱，超临界二氧化碳和甲醇。可以在210 nm处或通过MS将化合物分配到提取物中，无需额外的鞘液（甲醇中的0.1％乙酸）进行修饰。确定的木脂素模式是典型的中华链球菌，五味子酚A是最丰富的化合物，其次是五味子素B或五味子酚B。