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A novel surface-enhanced Raman scattering-based ratiometric approach for detection of hyaluronidase in urine.
Talanta ( IF 5.6 ) Pub Date : 2020-03-14 , DOI: 10.1016/j.talanta.2020.120915
Yanmei Si 1 , Lulu Li 2 , Binsheng He 3 , Jishan Li 1
Affiliation  

A ratiometric surface-enhanced Raman scattering (SERS) based method is described for the determination of the activity of hyaluronidase (HAase). Gold nanorods (AuNRs) were functionalized with 4-thiobenzonitrile (TBN) to act as the Raman reporter (TBN-AuNRs), and 4-thiophenylacetylene-functionalized gold-silver alloy nanoparticles (TPA-AuAgNPs) were used as the reference. Hyaluronic acid (HA) acts as the HAase recognition element. The TBN-modified AuNRs aggregate in the presence of HA due to the strong electrostatic interaction between the positively charged TBN-AuNRs and negatively charged HA. This strongly enhances the Raman signal of TBN at 2220 cm-1. However, HA has no significant effect on the dispersion of the modified AuAg NPs which are electroneutral. Hence, no change can be seen in the Raman intensity of TPA at 1974 cm-1. In the presence of HAase, HA is digested into smaller fragments. This results in good dispersion of the TBN-AuNRs and a weaker TBN Raman signal. Hence, the ratio of the Raman peaks at 1974 and 2220 cm-1 increases. Under the optimized conditions, the ratio changes in the 5-70 U mL-1 HAase activity range, and the detection limit is 1.7 U mL-1 (based on the 3σ rule). Moreover, this method has been successfully applied in the determination of the activity of HAase in artificial urine and it is expected to be a new method for the diagnosis of cancer, especially bladder cancer.

中文翻译:

一种新的基于表面增强拉曼散射的比例测定方法,用于检测尿液中的透明质酸酶。

描述了一种基于比例表面增强拉曼散射(SERS)的方法,用于测定透明质酸酶(HAase)的活性。金纳米棒(AuNRs)用4-硫代苄腈(TBN)功能化以充当拉曼报告分子(TBN-AuNRs),并且使用4-硫代苯乙炔官能化的金银合金纳米粒子(TPA-AuAgNPs)作为参考。透明质酸(HA)充当HAase识别元件。由于带正电的TBN-AuNR和带负电的HA之间存在强烈的静电相互作用,因此TBN修饰的AuNR在HA存在下聚集。这极大地增强了2220 cm-1处TBN的拉曼信号。但是,HA对电子中性的修饰AuAg NP的分散没有明显影响。因此,在1974 cm-1处TPA的拉曼强度没有变化。在存在HAase的情况下,HA被消化成较小的片段。这导致TBN-AuNRs的良好分散和较弱的TBN拉曼信号。因此,1974年和2220 cm-1处拉曼峰的比率增加。在优化的条件下,该比率在5-70 U mL-1 HAase活性范围内变化,检出限为1.7 U mL-1(基于3σ规则)。此外,该方法已成功地用于测定人造尿中HAase的活性,有望成为诊断癌症,尤其是膀胱癌的新方法。比率在5-70 U mL-1 HAase活性范围内变化,检出限为1.7 U mL-1(基于3σ规则)。而且,该方法已经成功地用于测定人造尿中HAase的活性,有望成为诊断癌症,尤其是膀胱癌的新方法。比率在5-70 U mL-1 HAase活性范围内变化,检出限为1.7 U mL-1(基于3σ规则)。此外,该方法已成功地用于测定人造尿中HAase的活性,有望成为诊断癌症,尤其是膀胱癌的新方法。
更新日期:2020-03-16
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