Bioimaging probes for monitoring intracellular reactive oxygen species have important implications for cell biology research. Herein, we developed peptide-capped silver/gold nanoclusters (peptide@Ag/Au NCs) for lysosome-targeted imaging of hypochlorite (ClO⁻). The peptide@Ag/Au NCs were synthesized via a one-pot method using peptide as both a template and a reducing agent. The fluorescence intensity and absolute quantum yield of peptide@Ag/Au NCs were much higher than those of peptide-capped gold nanoclusters and silver nanoclusters. In the presence of ClO⁻, the fluorescence of peptide@Ag/Au NCs was quenched, accompanied by a redshift due to ClO⁻-induced oxidation of the peptide ligand and decreased Ag content in Ag/Au NCs. The relative fluorescence intensity F₀/F had favourable linearity for ClO⁻ concentrations in the range 0.1–100 μmol/L (R² = 0.9954), with a detection limit (LOD) of 80 nmol/L. The lysosome-targeted peptide@Ag/Au NCs were applied to detect ClO⁻ in lysosomes in living cells via fluorescence imaging.

用于监视细胞内活性氧种类的生物成像探针对细胞生物学研究具有重要意义。在此，我们开发了用于次氯酸盐的溶酶体靶向成像肽-封端的银/金纳米簇（肽@的Ag / Au的NCS）（CLO ^- ）。通过使用肽作为模板和还原剂两者的一锅法合成肽@ Ag / Au NC。肽@ Ag / Au NCs的荧光强度和绝对量子产率比肽封端的金纳米团簇和银纳米团簇高。在CLO的存在^- ，肽@的Ag / Au的NC的荧光猝灭，伴随有由于红移至C10 ^-诱导的肽配体氧化，降低了Ag / Au NCs中的Ag含量。相对荧光强度˚F ₀ / F有用于CLO有利线性^-浓度范围为0.1〜100微摩尔/ L（R ² = 0.9954），以80 nmol / L的检测限（LOD）。溶酶体靶向肽@的Ag / Au的纳米晶被应用到检测CLO ^-在溶酶体中通过荧光成像活细胞。