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NIR fluorescent probe based on a modified rhodol-dye with good water solubility and large Stokes shift for monitoring CO in living systems.
Talanta ( IF 5.6 ) Pub Date : 2020-03-14 , DOI: 10.1016/j.talanta.2020.120914
Jiaxin Hong 1 , Qingfeng Xia 1 , Enbo Zhou 1 , Guoqiang Feng 1
Affiliation  

Because of the biological importance of CO, the development of effective probes for the detection of CO in living systems is of great significance. In particular, near-infrared (NIR) fluorescence probes with good water solubility and large Stokes shift are indispensable tools for CO detection in vivo. However, such tools are extremely scarce. Herein, a modified rhodol dye was used to develop a novel NIR fluorescent probe (Rh-NIR-CO) for selective and sensitive detection of CO using the Pd0-mediated Tsuji-Trost reaction. This probe shows good water solubility and rapid CO detecting ability in aqueous buffer at pH 7.4, accompanied by distinct colorimetric and turn-on NIR emission changes at 676 nm with a large Stokes shift (135 nm) and low detection limit (37 nM). Moreover, NIR fluorescence imaging of CO in living cells, zebrafish, and living mice was successfully applied with this probe. These excellent results highlighted Rh-NIR-CO as a promising new tool for in vitro and in vivo detection of CO.

中文翻译:

NIR荧光探针基于改良的Rhodol染料,具有良好的水溶性和较大的斯托克斯位移,可用于监测生命系统中的CO。

由于CO的生物学重要性,开发用于检测生命系统中CO的有效探针具有重要意义。特别地,具有良好的水溶性和大斯托克斯位移的近红外(NIR)荧光探针是体内CO检测不可缺少的工具。但是,此类工具极为稀缺。在本文中,改性的rhodol染料用于开发一种新型的NIR荧光探针(Rh-NIR-CO),该探针使用Pd0介导的Tsuji-Trost反应选择性和灵敏地检测CO。该探针在pH 7.4的水性缓冲液中显示出良好的水溶性和快速的CO检测能力,并伴随着在676 nm处明显的比色和开启NIR发射变化,并具有较大的斯托克斯位移(135 nm)和低检测限(37 nM)。此外,在活细胞,斑马鱼,并成功地将活小鼠应用于该探针。这些出色的结果突显了Rh-NIR-CO是一种有前途的体外和体内CO检测新工具。
更新日期:2020-03-16
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