During the last years, enzyme-based biosensors have gained much more attention among the researchers and have had great success in the determination of different biological macromolecules. Nanomaterials with intrinsic enzyme-mimic activity are widely used in biomedicine as artificial enzymes. Here, we report glucose oxidase-mimic activity of nanolayered manganese-calcium (Mn-Ca) oxide nanoparticles (NL-MnCaO2). In this work, NL-MnCaO2nanoparticles were synthesized and characterized using different techniques including transmission electron microscopy (TEM), scanning electron microscopy (SEM), fourier-transform infrared spectroscopy (FTIR) and powder X-ray diffraction (XRD). Also, the ability of these compounds for the glucose and hydrogen peroxide (H2O2) determination was investigated. A non-enzymatic strategy for the colorimetric detection of glucose and H2O2 was reported which can be utilized not only for the rapid detection and analysis of glucose by the naked eye but also the quantitative assay of glucose by spectrophotometry. The in situ generated H2O2 and gluconic acid (GA) from the oxidation of glucose through the glucose oxidase-mimicking activity of NL-MnCaO2 was detected using a colorimetric method. Also, the results confirmed the application of these compounds for the detection of glucose in human serum samples with a detection limit (LOD) of 6.12 × 10-6 M. The results showed that NL-MnCaO2 can be used as an alternative for the natural enzymes and act as a simple, sensitive and enzyme-free biosensor for the detection of glucose in real samples. The proposed strategy shows some advantages including sensitivity, short detection time and low detection limit.

中文翻译：

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基于纳米层状锰钙氧化物的葡萄糖氧化酶样活性的用于检测葡萄糖的超灵敏无标记比色生物传感器

近年来，基于酶的生物传感器越来越受到研究人员的关注，并在测定不同生物大分子方面取得了巨大成功。具有内在酶模拟活性的纳米材料作为人工酶广泛应用于生物医学。在这里，我们报告了纳米层状锰钙（Mn-Ca）氧化物纳米颗粒（NL-MnCaO2）的葡萄糖氧化酶模拟活性。在这项工作中，NL-MnCaO2 纳米颗粒的合成和表征使用不同的技术，包括透射电子显微镜 (TEM)、扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR) 和粉末 X 射线衍射 (XRD)。此外，还研究了这些化合物测定葡萄糖和过氧化氢 (H2O2) 的能力。报道了一种用于比色检测葡萄糖和 H2O2 的非酶促策略，该策略不仅可用于肉眼快速检测和分析葡萄糖，还可用于分光光度法定量测定葡萄糖。使用比色法检测葡萄糖氧化通过 NL-MnCaO2 的葡萄糖氧化酶模拟活性原位生成的 H2O2 和葡萄糖酸 (GA)。此外，结果证实了这些化合物可用于检测人血清样品中的葡萄糖，检测限 (LOD) 为 6.12 × 10-6 M。结果表明 NL-MnCaO2 可作为天然酶作为一种简单、灵敏且无酶的生物传感器，用于检测实际样品中的葡萄糖。所提出的策略显示出一些优势，包括灵敏度、