Optical detection setup utilizing light emitting diodes (LEDs), 50 nL L-shaped silica capillary detection cell (L-cell), and low-cost CCD spectrometer is described in this work. Experimental configuration can be equipped with two different LEDs for absorbance measurement and other two LEDs for fluorescence excitation. This setup is capable of simultaneous multi-wavelength monitoring of absorbance and fluorescence when light produced by the individual LEDs and light emitted by the fluorescent analytes is resolved in the spectrum outputted by the CCD spectrometer. Effective optical path of the 0.25 μm I. D. L-cell is 1 mm. Absorbance baseline noise is 1 mAU due to use of low-cost and relatively noisy CCD spectrometer and LED drivers. Nevertheless, the setup can detect adenosine 5'-monophosphate down to micromolar concentration. Performance of fluorescence monitoring allows detection of 5·10-10 M fluorescein when 23 mW 470 nm LED is used for excitation. The dynamic range of absorbance and fluorescence measurement is 867:1 and 1622:1, respectively. Separation of test mixture (alkylbenzenes and polyaromatic hydrocarbons) demonstrate the effective use of the detector for simultaneous absorbance and fluorescence detection with 0.2 × 150 mm packed capillary column. The benefits of the setup are relative simplicity, compact design and the fact that it can be operated without any optical filters, slits, and extremely precise positioning of the optical elements.

中文翻译：

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紧凑型光学检测器采用发光二极管、50 nL L 形硅胶毛细管和 CCD 光谱仪，可在微柱液相色谱中同时多波长监测吸光度和荧光

在这项工作中描述了利用发光二极管 (LED)、50 nL L 形二氧化硅毛细管检测单元 (L 单元) 和低成本 CCD 光谱仪的光学检测设置。实验配置可以配备两个不同的 LED 用于吸光度测量，另外两个 LED 用于荧光激发。当单个 LED 产生的光和荧光分析物发出的光在 CCD 光谱仪输出的光谱中解析时，该设置能够同时对吸光度和荧光进行多波长监测。0.25 μm ID L-cell 的有效光路为 1 mm。由于使用低成本且噪声相对较大的 CCD 光谱仪和 LED 驱动器，吸光度基线噪声为 1 mAU。然而，该设置可以检测低至微摩尔浓度的腺苷 5'-单磷酸。当 23 mW 470 nm LED 用于激发时，荧光监测的性能允许检测 5·10-10 M 荧光素。吸光度和荧光测量的动态范围分别为 867:1 和 1622:1。测试混合物（烷基苯和多环芳烃）的分离表明检测器可以有效地使用 0.2 × 150 mm 填充毛细管柱同时进行吸光度和荧光检测。该装置的优点是相对简单、设计紧凑，并且无需任何滤光片、狭缝和光学元件的极其精确定位即可操作。测试混合物（烷基苯和多环芳烃）的分离表明检测器可以有效地使用 0.2 × 150 mm 填充毛细管柱同时进行吸光度和荧光检测。该装置的优点是相对简单、设计紧凑，并且无需任何滤光片、狭缝和光学元件的极其精确定位即可操作。测试混合物（烷基苯和多环芳烃）的分离表明检测器可以有效地使用 0.2 × 150 mm 填充毛细管柱同时进行吸光度和荧光检测。该装置的优点是相对简单、设计紧凑，并且无需任何滤光片、狭缝和光学元件的极其精确定位即可操作。