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Proteomic analysis of the phytogenic fungus Sclerotinia sclerotiorum.
Journal of Chromatography B ( IF 2.8 ) Pub Date : 2020-03-14 , DOI: 10.1016/j.jchromb.2020.122053
Sarah Otun 1 , Khayalethu Ntushelo 2
Affiliation  

Sclerotinia sclerotiorum is a necrotrophic phytopathogen that has been the subject of several scientific research efforts. Despite the numerous research efforts its proteome remains understudied. This study aimed to identify proteins produced by S. sclerotiorum, thereby increasing the current proteomic knowledge base. Total proteins were extracted from mycelia scraped from five-day old cultures of S. sclerotiorum. The extracted proteins were separated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) and were annotated using the AB Sciex TripleToF 6600 mass spectrometer. Exactly 1471 proteins were reproducibly present in all three replicates. All proteins detected were classified based on their molecular and biological functions. To the knowledge of the authors, this is the most comprehensive proteomic study on S. sclerotiorum (judging by the high number of proteins identified).

中文翻译:

植物菌根菌核盘菌的蛋白质组学分析。

核盘菌核盘菌是一种坏死性植物病原体,已经成为若干科学研究工作的主题。尽管进行了大量的研究工作,其蛋白质组学仍未被充分研究。这项研究旨在鉴定核盘菌产生的蛋白质,从而增加当前的蛋白质组学知识基础。从五天的葡萄球菌培养物中刮取的菌丝体中提取总蛋白。提取的蛋白质通过十二烷基聚丙烯酰胺钠凝胶电泳(SDS-PAGE)分离,并使用AB Sciex TripleToF 6600质谱仪标注。在所有三个重复中都精确地存在1471个蛋白质。根据检测到的所有蛋白质的分子和生物学功能对其进行分类。据作者所知,这是关于S的最全面的蛋白质组学研究。
更新日期:2020-03-16
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