The presented paper is the first to show thin layer chromatography (TLC) analysis based on eutectic mobile phases (Deep Eutectic Solvents - DES). During the experiment 25 eutectic mixtures were investigated for their chromatographic properties. Most of them belong to the natural deep eutectic solvents (NADES) group. Also, new eutectic liquids based on phenolics and terpenes, not previously employed in analytical practice, were tested. The eutectic liquids were investigated as pure or diluted with solvents used in chromatographic routine: methanol, water, acetone, chloroform or diethyl ether. The analyses were carried out using classic and high performance silica gel plates. The working solution was a mixture of five alkaloids found in genus Chelidonium, namely sanguinarine, coptisine, chelerythrine, chelidonine, and berberine, with UV light detection of 366 nm. This report proves that eutectic TLC is possible and that the eutectic interactions play a crucial role in the separation process. In most of the tested modifications at least partial separation was achieved. The most successful mobile phase, which enabled separation of all the tested alkaloids, was the equimolar mixture of menthol and phenol with a 35% addition of methanol. The system was also effective in separating alkaloids in the real Chelidonium maius extract sample.

中文翻译：

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具有共晶流动相的薄层色谱法初步结果。

这篇论文是第一篇展示基于共晶流动相（Deep Eutectic Solvents-DES）的薄层色谱（TLC）分析的论文。在实验过程中，研究了25种共晶混合物的色谱特性。它们大多数属于天然深共熔溶剂（NADES）组。此外，还测试了以前在分析实践中未使用的基于酚类和萜烯的新型共晶液体。研究低共熔液体的纯度，或用色谱法常规溶剂稀释：甲醇，水，丙酮，氯仿或乙醚。使用经典的高性能硅胶板进行分析。工作溶液是在白屈菜属中发现的五种生物碱的混合物，即血红碱，黄连，白屈菜红碱，白屈菜碱和小ber碱，366 nm的紫外线检测。该报告证明了共晶TLC是可能的，并且共晶相互作用在分离过程中起着至关重要的作用。在大多数测试的修改中，至少实现了部分分离。能够分离所有测试生物碱的最成功的流动相是薄荷醇和苯酚的等摩尔混合物，添加了35％的甲醇。该系统还可以有效分离真正白屈菜提取物样品中的生物碱。是薄荷醇和苯酚的等摩尔混合物，加了35％的甲醇。该系统还可以有效分离真正白屈菜提取物样品中的生物碱。是薄荷醇和苯酚的等摩尔混合物，加了35％的甲醇。该系统还可以有效分离真正白屈菜提取物样品中的生物碱。