In this work, an innovative method is described for multi-residue pesticide analysis by liquid chromatography coupled to targeted mass spectrometry, called "Scout-MRM, this new acquisition mode relies on the monitoring by either endogenous or spiked Scout compounds, hence fully releasing the monitoring of target molecules from time scheduling. As a proof of concept, a Scout-MRM method was built where 5 transitions groups tracking a total of 191 pesticides where successively triggered under the control of 5 spiked-in deuterated pesticides. As expected from its retention time independency, Scout-MRM demonstrates strong detection robustness towards modifications of gradient parameters, as well as easy method transfer between distinct analytical platforms with nearly 100% recovery after a single run. Finally, Scout-MRM was used for the multi-residue screening and quantification of pesticides in real surface water samples, by applying an external calibration procedure and comparing it with classical scheduled reaction monitoring methods.

中文翻译：

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侦察多反应监测：液相色谱串联质谱法，无需时间表即可进行多残留农药分析。

在这项工作中，描述了一种创新的方法，该方法通过液相色谱与目标质谱联用的液相色谱法进行多残留农药分析，称为“ Scout-MRM，这种新的采集模式依赖于通过内源或加标的Scout化合物进行监测，从而完全释放出从时间安排上监视目标分子作为概念证明，建立了Scout-MRM方法，其中5个过渡小组跟踪了总共191种农药，并在5种加标的氘化农药的控制下连续触发。与时间无关，Scout-MRM表现出对梯度参数修改的强大检测鲁棒性，并且易于在不同分析平台之间轻松进行方法转移，单次运行后回收率接近100％。通过应用外部校准程序并将其与经典的计划反应监测方法进行比较，Scout-MRM用于实际地表水样品中农药的多残留筛选和定量。