A fluorescent and low molecular weight rotor (9) based on 1, 8-napthalimide for living cancer cell imaging was designed and synthesized. The effects of solvent polarity and viscosity on the fluorescence spectra of compound 9 were investigated. Rotor 9 exhibited favorable photophysical properties with a large Stokes shift and induced high viscosity sensitivity in a glycerol-ethanol system with an approximately 10-fold increase in emission intensity. The addition of different concentrations of BSA to compound 9 in PBS elicited an 8-fold increase in fluorescence intensity, suggesting superior environmental sensitivity. Fluorescence lifetime measurements also confirmed these results. Furthermore, compound 9 showed time- and concentration-dependent in vitro cellular uptake behavior in MCF-7 cells and other cancer cell lines. CLSM assay of tumor sections from MCF-7 nude mice also confirmed the possibility of tumor imaging.

设计并合成了基于1，8-萘二酰亚胺的荧光低分子转子（9），用于活体癌细胞成像。研究了溶剂极性和粘度对化合物9荧光光谱的影响。转子9表现出良好的光物理特性，具有大的斯托克斯位移，并在甘油-乙醇系统中引起了高粘度敏感性，并且发射强度提高了约10倍。在PBS中向化合物9添加不同浓度的BSA，会引起荧光强度增加8倍，表明对环境的敏感性更高。荧光寿命测量也证实了这些结果。此外，化合物9在MCF-7细胞和其他癌细胞系中显示出时间依赖性和浓度依赖性的体外细胞摄取行为。来自MCF-7裸鼠的肿瘤切片的CLSM分析也证实了肿瘤成像的可能性。