The inherent biological inertness of Ti substrates is a general challenge in orthopedic and dental clinical application. In this study, the strontium-containing phase change lysozyme coating was prepared on titanium via a supramolecular self-assembly method for accelerating osseointegration. Successful preparation of the Sr-incorporated lysozyme nanofilm onto the Ti substrate (Ti-Ly-Sr) was proved by tests of scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and Contact angle (CA). Furthermore, the results of cell morphology observation, cell viability assay, alkaline phosphatase staining and quantitative analysis showed that Ti-Ly-Sr substrate enhanced early adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs). The quantitative real-time polymerase chain reaction (qRT-PCR) assays confirmed that Ti-Ly-Sr enhanced the expression of osteogenic related genes (BMP2, OPG, Runx2 and COL-1) of BMSCs at the molecular level. Moreover, Micro-CT and histological analysis proved that the strontium-incorporated lysozyme nanofilm modified Ti implants had significant capability of new bone formation in vivo after implantation for 4 weeks. We anticipate that the present system would provide a facile and effective means for surface modification of Ti-based implants. Relevant ideas and techniques in this study will contribute to the development of new implant devices.

Ti底物固有的生物学惰性是整形外科和牙科临床应用中的普遍挑战。在这项研究中，含钛的相变溶菌酶涂层是在钛上通过一种超分子自组装方法，用于加速骨整合。通过扫描电子显微镜（SEM），原子力显微镜（AFM），X射线光电子能谱（XPS）和接触角测试证明了成功地将掺有Sr的溶菌酶纳米膜制备到Ti基质（Ti-Ly-Sr）上。 （CA）。此外，细胞形态观察，细胞活力测定，碱性磷酸酶染色和定量分析的结果表明，Ti-Ly-Sr底物增强了骨髓基质细胞（BMSCs）的早期粘附，增殖和成骨分化。定量实时聚合酶链反应（qRT-PCR）分析证实，Ti-Ly-Sr在分子水平上增强了BMSCs成骨相关基因（BMP2，OPG，Runx2和COL-1）的表达。此外，植入后体内4周。我们期望本系统将提供用于钛基植入物的表面改性的简便有效的手段。这项研究中的相关思想和技术将有助于新型植入设备的开发。